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 摘要： [Objectives] To determine the content of Zhuang medicine Sauropus spatulifolius Beille from Guangxi. [Methods] The amino acid content of S. spatulifolius Beille was determined by ultraviolet spectrophotometry (UVs). The content of kaempferol-3-O-gentiobioside inS. spatulifolius  Beille was determined by liquid chromatography-mass spectrometry (LC-MS). Pesticide residues in S. spatulifolius Beille were detected by gas chromatography-mass spectrometry (GC-MS). Heavy metal elements arsenic (As), cadmium (Cd), and lead (Pb) in S. spatulifolius Beille were detected by inductively coupled plasma mass spectrometry (ICP-MS). [Results] The amino acid content in S. spatulifolius  Beille was 3.233 mg/g, with a relative standard deviation (RSD) of 0.36%. The content of kaempferol-3-O-gentiobioside was 1.15 μg/mL. No pesticide residues or heavy metals were detected in the S. spatulifolius  Beille medicinal material. [Conclusions] This study improves the quality control system for S. spatulifolius Beille and provides a reference basis for the quality standard control of Zhuang medicineS. spatulifolius  Beille from Guangxi.

 摘要： This paper provides a systematic review of the histochemical localization, content characteristics, and influencing factors of saponins in Pseudostellaria heterophylla based on an extensive literature survey. It provides an in-depth analysis and summary of the effects of biological factors, environmental conditions, agronomic practices, processing methods, and continuous cropping obstacles on the synthesis of P. heterophylla saponins, as well as their underlying mechanisms. Based on identified gaps in the current literature, future research directions and prospects are proposed. The findings of this review offer valuable insights for advancing the understanding of the saponin biosynthesis mechanisms in P. heterophylla and for enhancing its quality.

 摘要： [Objectives] To investigate the efficacy and potential mechanism of the topical preparation Jineijin-Shanzha Patch (composed of Galli Gigerii Endothelium Corneum and Crataegi Fructus) in improving functional dyspepsia (FD) based on network pharmacology. [Methods] Firstly, we reviewed the existing research progress on each constituent drug of the Jineijin Shanzha Patch for FD improvement. Following this, identified overlapping genes were utilized to construct both a "Drug-Active Component-FD Target" network and a Protein-Protein Interaction (PPI) network specific to the patch. In addition, Gene Ontology (GO) analysis was carried out. [Results] We identified that the 13 herbs in the Jineijin Shanzha Patch are mainly used for food stagnation, qi stagnation, and spleen deficiency. Screening revealed 43 active patch components, 1 414 FD-related targets, and 284 shared targets between them. The PPI network analysis further identified the top 10 core targets from these shared targets. From the "Drug-Active Component-FD Target" network, we identified the core elements. These included the herbal components Vignae Semen (from Liushenqu), Crataegi Fructus, and Pseudostellariae Radix; the active components quercetin, genistein, and apigenin; and the key targets CASP3, BCL2, and CASP9. GO analysis of the 284 overlapping targets indicated that the Jineijin Shanzha Patch may exert its therapeutic effects via regulation of biological processes such as the response to lipopolysaccharide, response to bacterium-derived molecules, and regulation of the apoptotic signaling pathway. [Conclusions] The main active components of the Jineijin Shanzha Patch (quercetin, genistein, and apigenin) may improve FD by modulating signaling pathways such as the response to lipopolysaccharide, response to bacterium-derived molecules, and regulation of the apoptotic signaling pathway, thereby acting on key targets including CASP3, BCL2, and CASP9.

 摘要： [Objectives] To evaluate the in vitro antibacterial, antioxidant, and α-glucosidase inhibitory activities of the ethanol total extract and four different polarity fractions (n-butanol, ethyl acetate, petroleum ether, and water) of Pilea peltata Hance, so as to provide a reference for its further development and research. [Methods] The antibacterial activity of P. peltata was evaluated in vitro by determining the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of its ethanol total extract and four different polarity fractions against seven test bacterial strains using the broth microdilution method. The in vitro antioxidant activity was investigated through DPPH radical, hydroxyl radical, and superoxide anion radical scavenging assays, with vitamin C (Vit C) as the positive control and the half maximal scavenging concentration (IC₅₀) as the evaluation indicator. The in vitro α-glucosidase inhibitory activity was assessed by measuring the peak area of p-nitrophenol (PNP), the hydrolysis product of 4-nitrophenyl α-D-glucopyranoside (PNPG), via high-performance liquid chromatography (HPLC), using the half maximal inhibitory concentration(IC₅₀) as the evaluation indicator. [Results] Both the ethanol total extract and the four different polarity fractions of P. peltata exhibited significant in vitro anti-Streptococcus pneumoniae activity. The DPPH radical scavenging capacities of the ethanol total extract and the various fractions were all weaker than that of VitC, with the order of efficacy being: n-butanol fraction > ethanol total extract > ethyl acetate fraction > petroleum ether fraction > aqueous fraction. For hydroxyl radical scavenging activity, the efficacy order of P. peltata fractions was: n-butanol extract > ethyl acetate extract > ethanol total extract > petroleum ether extract > aqueous extract. Notably, the n-butanol fraction (IC₅₀ = 0.068 ± 0.001) demonstrated stronger activity than VitC (IC₅₀= 0.097 ± 0.001). The activity of the ethyl acetate fraction (IC₅₀= 0.096 ± 0.004) was comparable to that of VitC (IC₅₀ = 0.097 ± 0.001). The superoxide anion scavenging capacities of the ethanol total extract and different polarity fractions from P. peltata were all weaker than that of VitC, with the order of efficacy being: n-butanol fraction > ethyl acetate fraction > ethanol total extract > petroleum ether fraction > aqueous fraction. The ethanol total extract and aqueous fraction of Pilea peltata showed no significant in vitro α-glucosidase inhibitory activity. Compared with the acarbose group, the IC₅₀ values of the ethyl acetate fraction and the n-butanol fraction both showed highly significant differences (P < 0.01). [Conclusions] This study provides an experimental basis for the pharmacodynamic study and active component study of P. peltata.

 摘要： [Objectives] To develop an HPLC fingerprint analysis method for the medicinal material of Lysimachia foenum-graecum Hance, thereby providing a foundation for its quality control. [Methods] Samples of L. foenum-graecum collected from 10 distinct locations in Guangxi were analyzed using HPLC, and chromatographic fingerprints were established. The Similarity Evaluation System for Chromatographic Fingerprint of Traditional Chinese Medicine (2012 Edition) was employed for common peak calibration and similarity evaluation. Additionally, principal component analysis was performed on the common peak area data. [Results] An HPLC fingerprint of L. foenum-graecum was developed, identifying a total of 13 common peaks. Among these, four characteristic components were specifically identified: chlorogenic acid, myricetin, quercetin, and kaempferol. The kaempferol chromatographic peak, exhibiting good resolution and a stable peak shape, was selected as the reference peak. The similarity indices between the fingerprints of the 10 sample batches and the reference fingerprint ranged from 0.954 to 0.995, indicating a relatively high consistency in the chemical composition of L. foenum-graecum from different origins. Principal component analysis identified two principal components, which together accounted for 89.45% of the cumulative variance, effectively capturing the primary chemical differences among the samples. [Conclusions] The established HPLC fingerprint method is straightforward to implement, stable, reliable, and exhibits high specificity. When combined with similarity evaluation and principal component analysis, it offers a scientific basis for developing quality standards for L. foenum-graecum medicinal materials.

 摘要： [Objectives] To assess the effects of endophytic bacteria on the growth, antioxidant responses, and and the production of key secondary metabolites in Emilia prenanthoidea DC. [Methods] Three endophytic strains (H1, H2, L1) were inoculated onto tissue-cultured seedlings and cultivated for 20 d under greenhouse conditions. Growth traits, reactive oxygen species (ROS) indicators, antioxidant enzyme activities, and the content of chlorogenic acid and quercetin were analyzed using one-way ANOVA followed by Tukey’s test. [Results] Bacterial inoculation significantly enhanced plant performance. Treatment H2 increased plant height by 27%, chlorophyll content by 73%, and fresh weight by 31%. Levels of ROS (O₂^-, H₂O₂) and MDA decreased markedly, whereas the activities of POD and CAT increased. Additionally, the content of chlorogenic acid and quercetin increased by up to 67% and 64%, respectively, with both H2 and L1 treatments showing the most pronounced effects. [Conclusions] Endophytic bacteria markedly improve growth, redox balance, and phenolic accumulation in E. prenanthoidea. Strain H2 represents a promising bioinoculant for improving the medicinal quality of this species.

 摘要： The existence of the “stomach-knee axis” and the potential independent association between “Helicobacter pylori (Hp) infection and knee osteoarthritis (KOA)” have been substantiated through multiple lines of evidence, including epidemiological studies of the “stomach-knee axis”, investigations into the pathological mechanisms linking “Hp infection and arthritis”, as well as research on co-treatment drugs and the anti-inflammatory mechanisms relevant to “gastritis-arthritis”. The investigation into “stomach and knee joint treatment” is grounded in the concept that the “stomach-knee axis” aligns with the “combined bi of body constituents and zang organs” theory. Clinical cases have demonstrated that therapeutic strategies of “stomach and knee joint treatment” can mitigate KOA damage by modulating the “stomach-knee axis” and eradicating Hp infection in the stomach. This study aims to contribute novel insights into the cross-system treatment of KOA. Further investigation into the “stomach-knee axis” may yield innovative strategies for the prevention and management of KOA, potentially through the eradication of Hp.

 摘要： [Objectives] To investigate the clinical efficacy of core stability training combined with conventional rehabilitation in the functional recovery of patients suffering from chronic low back pain. [Methods] A randomized controlled trial design was employed in this study. Ninety patients with chronic low back pain were recruited and randomly assigned to either a control group (n=45), which received conventional rehabilitation, or an experimental group (n=45), which received conventional rehabilitation combined with core stability training. Both groups underwent treatment for 6 weeks. Assessments were conducted using the visual analogue scale (VAS), Oswestry disability index (ODI), and finger-to-floor test prior to test, 6 weeks following treatment, and during the follow-up period, respectively. [Results] Prior to treatment, no statistically significant differences were observed between the two patient groups in terms of general information and various baseline measurements (P>0.05). Following 6 weeks of treatment and throughout the follow-up period, both groups demonstrated significant improvements in VAS scores, ODI scores, and lumbar anteflexion range of motion compared to baseline measurements (P<0.05). Notably, the magnitude of improvement in the experimental group exceeded that of the control group, with this inter-group difference reaching statistical significance (P<0.05). No serious adverse reactions were reported during the treatment process. [Conclusions] Core stability training combined with conventional rehabilitation can significantly enhance the alleviation of pain and functional impairments in patients suffering from chronic low back pain. This approach holds valuable implications for the optimization of rehabilitation treatment protocols.

 摘要： This paper summarizes the nursing experience of a child with acute fulminant myocarditis. Key nursing measures include establishing a multidisciplinary team to jointly formulate diagnosis and treatment plans; implementing refined volume management, anticoagulation management, and ventilator management during extracorporeal membrane oxygenation; providing personalized nutritional support for the child; and strengthening the prevention and management of complications. After active treatment and nursing care, the child recovered well and was successfully transferred out of the intensive care unit.