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 摘要： [Objectives] To investigate whether Ailanthone (AIL) could reverse cisplatin resistance in non-small cell lung cancer (NSCLC) by modulating autophagy pathways in A549/DDP cells. [Methods] Cisplatin-resistant A549/DDP cells were treated with AIL (0.6 μmol/L), cisplatin (50 μg/mL), or their combination. Cell proliferation was assessed by MTT, EdU and colony formation assays; migration by Transwell and wound healing assays; autophagy markers (P62, LC3B, Beclin1, ATG5) by Western blot; LC3B puncta by immunofluorescence; with rescue experiments using rapamycin. [Results] The AIL-cisplatin combination synergistically inhibited proliferation and migration, while downregulating P-gp and MVP. AIL significantly increased P62 accumulation while decreasing LC3B-II, Beclin1 and ATG5. Rapamycin reversed these effects, restoring viability and resistance markers. [Conclusions] AIL reverses cisplatin resistance in NSCLC by inhibiting autophagy through P62/LC3B regulation, offering a promising therapeutic strategy for refractory NSCLC.

 摘要： [Objectives] To study the network pharmacology-based anticancer effect of Ardisiacrispin B for colon cancer (CRC). [Methods] The chemical structure and molecular properties of Ardisiacrispin B were assessed via the PubChem resource, while the putative target genes of Ardisiacrispin B were predicted using the PharmMapper Database. Moreover, Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway and Gene Ontology (GO) enrichment analyses were conducted via the WebGestalt platform. Finally, a drug-target-pathway network was built via Cytoscape to show the visual representation. [Results] Ardisiacrispin B exhibited exceptional druggability with 25 putative targets. Analyses conducted using KEGG, GO, and network methods showed that these target genes were related with inflammatory responses, cancer, and varoius other biological functions. On the basis of these findings, we further screened the correlative biotargets of Ardisiacrispin B in relation to CRC, and explored the anticancer effects of Ardisiacrispin B for the treatment of CRC through CCK8 analysis and colony formation assay. Our results confirmed that Ardisiacrispin B exhibited anti-CRC properties, and suggested 11 candidate targets of Ardisiacrispin B in the treatment of CRC. [Conclusions] Ardisiacrispin B has been demonstrated to target multiple proteins/genes and pathways, thereby forming a network that displays systematic pharmacological activities. Moreover, it has potential therapeutic value in tumor treatment, specifically in promoting the proliferation of CRC cells.

 摘要： [Objectives] To investigate the potential applications and mechanisms of action of medicinal plants as bacteriostatic agents, utilizing snow lotus as a case study through network pharmacology. [Methods] The TCMSP and HIT 2.0 databases were employed to screen and obtain the active components and corresponding targets of snow lotus. The identified targets were subsequently intersected with the antibacterial and bacteriostatic targets sourced from the GeneCards and OMIM databases, resulting in the identification of the antibacterial and bacteriostatic targets associated with snow lotus. Cytoscape software was employed to construct the network diagram illustrating the active components and their corresponding action targets for snow lotus, as well as to analyze the network’s topology. Additionally, GO enrichment analysis of the action targets was conducted utilizing the DAVID database. [Results] A total of 12 active components of snow lotus were identified through screening, which corresponded to 294 action targets. Subsequent analysis revealed 117 core action targets of snow lotus that exhibit antibacterial and bacteriostatic properties. The results from the network diagram suggested that snow lotus may exert its antibacterial and bacteriostatic effects through active components such as quercetin, apigenin, and luteolin. Additionally, it appeared to activate the immunomodulatory functions of the human body by interacting with targets such as CASP3, TNF, and IL-6. [Conclusions] Snow lotus may demonstrate antibacterial and bacteriostatic properties through mechanisms of action that involve multiple components, targets, and pathways, from in vivo and in vitro multiple pathways. The integration of botanical bacteriostatic agents with chemical disinfectants that possess broad-spectrum bactericidal effects is advantageous for broadening the bactericidal spectrum and minimizing irritation, in order to facilitate the development of more environmentally friendly and low-toxicity disinfection and bacteriostatic products.

 摘要： [Objectives] To investigate the impact of aluminium on secretase activity. [Methods] A total of 60 Kunming mice were divided into three groups: a control group, a medium-dose model group, and a high-dose model group, with 20 mice in each group. All groups, except for the control group, received sequential injections of aluminum maltolate. The dosage for each injection was 0.3 and 0.45 mL per mouse, administered once daily. An injection pause of 2 d was observed every 5 d throughout the duration of the experiment, which lasted for a total of 40 d. At the conclusion of the study, blood samples were collected to isolate serum, and the brains were taken to prepare a 10% brain homogenate for subsequent analysis. [Results] The activities of brain β-secretase were 11.08±1.65, 11.94±1.37▲, and 12.32±0.93▲ u/L, respectively, with an F-value of 4.290 and a P-value of 0.018. When compared to the control group, the differences were statistically significant, as indicated by ▲P<0.05. The activities of α-secretase were 23.02±3.52, 19.04±1.10▲, and 18.44±1.40▲▲ u/L, respectively, yielding an F-value of 5.972 and a P-value of 0.016. Comparisons with the control group revealed statistically significant differences, as denoted by ▲P<0.05 and ▲▲P<0.01. The activities of γ-secretase were 11.01±2.05▲▲, 10.38±1.94▲, and 9.00±1.62 u/L, respectively, with an F-value of 5.780 and a P-value of 0.005. When compared to the high-dose group, the differences were statistically significant, with ▲P<0.05 and ▲▲P<0.01. [Conclusions] Aluminium exerts a considerable influence on the activities of brain β, α, and γ-secretases.

 摘要： [Objectives] To predict core targets and pathways of flavonoids from Scutellaria baicalensis against PD via network pharmacology. [Methods] Network pharmacology was employed to predict targets of six flavonoids (baicalein, baicalin, chrysin, wogonin, wogonoside, oroxylin A) from S. baicalensis. PD-related targets were screened from DrugBank, DisGeNET, GeneCards, and NCBI databases. Compound-target-disease networks and protein-protein interaction (PPI) networks were constructed. Functional enrichment analysis (GO/KEGG) was performed via Metascape. Molecular docking (Autodock Vina) validated ligand-target binding affinities. [Results] Intersection analysis identified 18 pivotal targets from 148 compound targets and 18 PD-associated targets. PPI network analysis revealed PTGS2, ESR1, TNF, and ABCB1 as core targets (degree >6). KEGG enrichment highlighted ovarian steroidogenesis (hsa04913) and ABC transporters. Molecular docking confirmed robust binding between flavonoids and PTGS2 (binding energy<-5 kcal/mol; baicalin: -13.2). [Conclusions] Flavonoids synergistically target PTGS2/ESR1-mediated prostaglandin synthesis and hormonal pathways.

 摘要： [Objectives] To explore the methods for identifying pure honey. [Methods] Using 35 batches of Jiulongteng honey sourced from various production areas in Guangxi as the research subjects, this study investigated the chromogenic reactions of starch and dextrin, as well as the comparative study of thin-layer chromatography of oligosaccharides present in Jiulongteng honey. [Results] None of the 35 batches of Jiulongteng honey samples exhibited blue (indicating starch), green, or reddish-brown (indicating dextrin) coloration, suggesting that no adulterants such as artificially added starch, dextrin, or sugar were present in these samples. Furthermore, none of the 35 batches displayed additional spots below the corresponding positions of the control, indicating that the sugar composition was consistent with the oligosaccharide profile of natural honey. No components inconsistent with the oligosaccharide profile of natural honey were detected. Therefore, it can be concluded that the Jiulongteng honey samples in this experiment were pure and free from adulteration with starch, dextrin, or other sugar substances. [Conclusions] The method employed in this experiment is straightforward and quick to implement, effectively preventing adulterated honey from entering the market. It enhances the efficiency of quality control for Jiulongteng honey and promotes the healthy development of the Jiulongteng honey industry.

 摘要： In response to the challenges associated with the traditional synthesis process of hymenidin, such as complex reaction steps, low yields, high costs, and environmental concerns, the synthesis process has been significantly enhanced by optimizing reaction conditions, screening for efficient catalysts, and incorporating the concepts of green chemistry. The optimized process has significantly improved the synthesis efficiency and product quality of hymenidin, reduced production costs, and minimized environmental pollution, thereby providing robust support for its industrial production and broad application.

 摘要： [Objectives] To investigate the protective effects of Siwu Xuanhu decoction on the liver of mice with acute liver injury induced by CCl₄. [Methods] A total of 48 ICR mice were randomly assigned to six groups: a blank control group, a model group, a low dose group of Siwu Xuanhu decoction (100 mg/kg), a medium dose group of Siwu Xuanhu decoction (200 mg/kg), a high dose group of Siwu Xuanhu decoction (300 mg/kg), and a positive control group (silymarin 2 mg/kg). Each group comprised 8 mice. Each dosing group received the designated dose of the drug (10 mL/kg) via continuous gavage, while the blank group and the model group were administered an equivalent volume of normal saline for four weeks, three times per week. An acute liver injury model was established through the intraperitoneal administration of a 20% CCl₄ olive oil solution at a dosage of 2 mL/kg in all experimental groups, with the exception of the control group, which received an equivalent volume of the olive oil solution. After 24 h fasting with water intake, blood samples were collected from the ocular region. The blood samples were allowed to stand and subsequently subjected to centrifugation to isolate the upper layer of serum, and a diagnostic kit was employed to measure the levels of aspartate aminotransferase (AST), alanine aminotransferase (ALT) and interleukin-6 (IL-6), and the activity of superoxide dismutase (SOD) in the serum. [Results] In comparison to the control group, serum levels of AST and ALT were significantly elevated in the model group. Furthermore, when compared to the model group, treatment with Siwu Xuanhu decoction resulted in a reduction of serum levels of ALT, AST, and IL-6, while simultaneously increasing SOD activities. [Conclusions] Siwu Xuanhu decoction exhibits a protective effect against acute liver injury induced by CCl₄ in mice.

 摘要： [Objectives] To observe the effects of Huanglian Jiedu Decoction on pain behaviors in rat models of postherpetic neuralgia (PHN) and analyze potential mechanisms. [Methods] Twenty SD rats with PHN models induced by intraplantar injection of 50 μL (6×10⁶) varicella-zoster virus (VZV) into the left hind paw were randomly divided into two groups (n=10 each) using a random number table: the model group (PHN group) and the Huanglian Jiedu Decoction group. From day 7 after inoculation, the Huanglian Jiedu Decoction group received oral gavage of Huanglian Jiedu Decoction twice daily for 14 days, while the PHN group received an equal volume of 0.9% saline. Thermal withdrawal latency (TWL) and mechanical withdrawal threshold (MWT) were measured at 1, 4, 7, 14, and 21 d after inoculation. Pain-related behaviors (vocalization, scratching, and licking/biting of the hind paw) were recorded. [Results] Compared with the PHN group, the Huanglian Jiedu Decoction group exhibited significantly prolonged TWL and increased MWT at days 14 and 21 (P<0.05). At day 4 post-inoculation, both groups showed marked pain-related behaviors (e.g., vocalization, scratching, licking/biting). These behaviors persisted until day 21 in the PHN group but significantly decreased in the Huanglian Jiedu Decoction group at days 14 and 21 (P<0.05). [Conclusions] Huanglian Jiedu Decoction reduces pain sensitivity and alleviates pain-related behaviors in PHN model rats. The mechanism may involve elevating pain thresholds and decreasing pain conduction velocity.