| 刊名 | Medicinal Plant |
| 作者 | Guilin YANG, Qiji ZHOU, Chengtong LIU, Weimei HE, Lixiang LU, Xueping WEI, Lizhen LIN, Xinying MO* |
| 作者单位 | Affiliated Hospital of Youjiang Medical University for Nationalities, Baise 533000, China; Guangxi Key Laboratory of Artificial Intelligence for Genetic Diseases of Long-dwelling Nationalities, Baise 533000, China; Key Laboratory of Research on Prevention and Control of High Incidence Diseases in Western Guangxi, Baise 533000, China |
| DOI | DOI:10.19600/j.cnki.issn2152-3924.2026.010.005 |
| 年份 | 2026 |
| 刊期 | 1 |
| 页码 | 24-29 |
| 关键词 | Lysimachia foenum-graecum Hance, HPLC, Fingerprint, Similarity evaluation, Principal component analysis |
| 摘要 | [Objectives] To develop an HPLC fingerprint analysis method for the medicinal material of Lysimachia foenum-graecum Hance, thereby providing a foundation for its quality control. [Methods] Samples of L. foenum-graecum collected from 10 distinct locations in Guangxi were analyzed using HPLC, and chromatographic fingerprints were established. The Similarity Evaluation System for Chromatographic Fingerprint of Traditional Chinese Medicine (2012 Edition) was employed for common peak calibration and similarity evaluation. Additionally, principal component analysis was performed on the common peak area data. [Results] An HPLC fingerprint of L. foenum-graecum was developed, identifying a total of 13 common peaks. Among these, four characteristic components were specifically identified: chlorogenic acid, myricetin, quercetin, and kaempferol. The kaempferol chromatographic peak, exhibiting good resolution and a stable peak shape, was selected as the reference peak. The similarity indices between the fingerprints of the 10 sample batches and the reference fingerprint ranged from 0.954 to 0.995, indicating a relatively high consistency in the chemical composition of L. foenum-graecum from different origins. Principal component analysis identified two principal components, which together accounted for 89.45% of the cumulative variance, effectively capturing the primary chemical differences among the samples. [Conclusions] The established HPLC fingerprint method is straightforward to implement, stable, reliable, and exhibits high specificity. When combined with similarity evaluation and principal component analysis, it offers a scientific basis for developing quality standards for L. foenum-graecum medicinal materials. |
