| 摘要 |
[Objectives] To investigate the efficacy and potential mechanism of the topical preparation Jineijin-Shanzha Patch (composed of Galli Gigerii Endothelium Corneum and Crataegi Fructus) in improving functional dyspepsia (FD) based on network pharmacology. [Methods] Firstly, we reviewed the existing research progress on each constituent drug of the Jineijin Shanzha Patch for FD improvement. Following this, identified overlapping genes were utilized to construct both a "Drug-Active Component-FD Target" network and a Protein-Protein Interaction (PPI) network specific to the patch. In addition, Gene Ontology (GO) analysis was carried out. [Results] We identified that the 13 herbs in the Jineijin Shanzha Patch are mainly used for food stagnation, qi stagnation, and spleen deficiency. Screening revealed 43 active patch components, 1 414 FD-related targets, and 284 shared targets between them. The PPI network analysis further identified the top 10 core targets from these shared targets. From the "Drug-Active Component-FD Target" network, we identified the core elements. These included the herbal components Vignae Semen (from Liushenqu), Crataegi Fructus, and Pseudostellariae Radix; the active components quercetin, genistein, and apigenin; and the key targets CASP3, BCL2, and CASP9. GO analysis of the 284 overlapping targets indicated that the Jineijin Shanzha Patch may exert its therapeutic effects via regulation of biological processes such as the response to lipopolysaccharide, response to bacterium-derived molecules, and regulation of the apoptotic signaling pathway. [Conclusions] The main active components of the Jineijin Shanzha Patch (quercetin, genistein, and apigenin) may improve FD by modulating signaling pathways such as the response to lipopolysaccharide, response to bacterium-derived molecules, and regulation of the apoptotic signaling pathway, thereby acting on key targets including CASP3, BCL2, and CASP9. |
