| 刊名 | Medicinal Plant |
| 作者 | Zhiying WEI1,2, Junqi LI3, Xiaojiao PAN2, Fangchan LI2, Yaheng QIU2, Jiao JIANG2, Xuehuan CAO4* |
| 作者单位 | 1. Ruikang Hospital Affiliated to Guangxi University of Chinese Medicine, Nanning 530200, China; 2. Guangxi University of Chinese Medicine, Nanning 530200, China; 3. Faculty of Chinese Medicine Science, Guangxi University of Chinese Medicine, Nanning 530200, China; 4. Maternity and Child Health Care of Guangxi Zhuang Autonomous Region, Nanning 530005, China |
| DOI | DOI:10.19600/j.cnki.issn2152-3924.2026.010.004 |
| 年份 | 2026 |
| 刊期 | 1 |
| 页码 | 20-23，29 |
| 关键词 | Pilea peltata Hance, Antibacterial, Antioxidation, α-glucosidase inhibition |
| 摘要 | [Objectives] To evaluate the in vitro antibacterial, antioxidant, and α-glucosidase inhibitory activities of the ethanol total extract and four different polarity fractions (n-butanol, ethyl acetate, petroleum ether, and water) of Pilea peltata Hance, so as to provide a reference for its further development and research. [Methods] The antibacterial activity of P. peltata was evaluated in vitro by determining the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of its ethanol total extract and four different polarity fractions against seven test bacterial strains using the broth microdilution method. The in vitro antioxidant activity was investigated through DPPH radical, hydroxyl radical, and superoxide anion radical scavenging assays, with vitamin C (Vit C) as the positive control and the half maximal scavenging concentration (IC50) as the evaluation indicator. The in vitro α-glucosidase inhibitory activity was assessed by measuring the peak area of p-nitrophenol (PNP), the hydrolysis product of 4-nitrophenyl α-D-glucopyranoside (PNPG), via high-performance liquid chromatography (HPLC), using the half maximal inhibitory concentration(IC50) as the evaluation indicator. [Results] Both the ethanol total extract and the four different polarity fractions of P. peltata exhibited significant in vitro anti-Streptococcus pneumoniae activity. The DPPH radical scavenging capacities of the ethanol total extract and the various fractions were all weaker than that of VitC, with the order of efficacy being: n-butanol fraction > ethanol total extract > ethyl acetate fraction > petroleum ether fraction > aqueous fraction. For hydroxyl radical scavenging activity, the efficacy order of P. peltata fractions was: n-butanol extract > ethyl acetate extract > ethanol total extract > petroleum ether extract > aqueous extract. Notably, the n-butanol fraction (IC50 = 0.068 ± 0.001) demonstrated stronger activity than VitC (IC50= 0.097 ± 0.001). The activity of the ethyl acetate fraction (IC50= 0.096 ± 0.004) was comparable to that of VitC (IC50 = 0.097 ± 0.001). The superoxide anion scavenging capacities of the ethanol total extract and different polarity fractions from P. peltata were all weaker than that of VitC, with the order of efficacy being: n-butanol fraction > ethyl acetate fraction > ethanol total extract > petroleum ether fraction > aqueous fraction. The ethanol total extract and aqueous fraction of Pilea peltata showed no significant in vitro α-glucosidase inhibitory activity. Compared with the acarbose group, the IC50 values of the ethyl acetate fraction and the n-butanol fraction both showed highly significant differences (P < 0.01). [Conclusions] This study provides an experimental basis for the pharmacodynamic study and active component study of P. peltata. |
