A Preliminary Investigation into the Mechanism of Lipoxygenase in Responding to Pathogenic Stress in Physcomitrella patens Induced by Botrytis cinerea
摘要:
[Objectives] To investigate the mechanism of lipoxygenase in responding to pathogenic stress in Physcomitrella patens induced by Botrytis cinerea. [Methods] It used wild-type and NDGA-treated Physcomitrella patens as experimental materials to measure the activities of phenylalanine ammonia-lyase (PAL), chalcone synthase (CHS), and cinnamyl alcohol dehydrogenase (CAD), as well as the contents of SA, ABA, and ET after inoculation with Botrytis cinerea (0, 6, 12, 24, 48, 72 h). [Results] In wild-type plants inoculated with B. cinerea, the activities of CHS, PAL, and CAD increased during the early defense stage compared with the control (uninoculated wild-type). In NDGA-treated P. patens inoculated with B. cinerea, the levels of CHS, PAL, and CAD were lower than those in wild-type plants at the corresponding time points after inoculation, indicating a correlation between LOX activity and the changes in CHS, PAL, and CAD activities during early defense. The contents of SA, ABA, and ET in wild-type plants inoculated with B. cinerea were higher than those in the control (uninoculated wild-type). In NDGA-treated P. patens inoculated with B. cinerea, the levels of SA, ABA, and ET were lower than those in wild-type plants at the corresponding time points after inoculation, suggesting that the synthesis of SA, ABA, and ET is correlated with LOX activity during early defense. [Conclusions] This study will lay a foundation for further molecular-level research on the disease resistance mechanisms of LOX in bryophytes.
关键词:
Physcomitrella patens, Botrytis cinerea, Lipoxygenase, Resistance mechanism