 摘要： Kinesins are a superfamily of proteins widely present in eukaryotes, playing crucial roles in plant cell wall assembly, cell elongation regulation, gravity sensing, and fertility control. In this study, bioinformatics analysis of the OsKMP2 gene (LOC_Os02g28850) was performed using online tools such as ExPASy-ProtParam, ProtScale, CD-search, and DNAMAN software. Additionally, qRT-PCR was employed to analyze the tissue expression pattern of OsKMP2. The results showed that the molecular weight of the OsKMP2 is 118.397 28 kDa, and it is a hydrophilic and unstable acidic protein. Secondary structure prediction revealed that it primarily consists of α-helices (69.45%), random coils (25.19%), and extended strands (5.36%). The gene was expressed in various rice tissues, with the highest expression level observed in leaves. These results indicate that the OsKMP2 gene exhibits high evolutionary conservation and functional diversity in rice. 

 摘要： [Objectives] This study was conducted to rapidly qualitatively and quantitatively analyze the residues of 10 plant growth regulators (PGRs) in bean sprouts. [Methods] Using bean sprouts as the test material, a high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) method was established to determine the residual levels of 10 PGRs in bean sprouts. [Results] Under optimized conditions, the retention time of the 10 PGRs ranged from 6.45 to 11.43 min. When the mass concentration ranged from 0.005 to 0.05 μg/ml, all PGRs exhibited good linearity, with correlation coefficients (r)≥0.999 1. The limits of detection (LODs, S/N=3) were in the range of 0.30-0.92 μg/kg, and the limits of quantification (LOQs) were  in the range of 0.50-2.10 μg/kg. The average recovery values at three concentration levels ranged from 80% to 105.8%, with relative standard deviations (RSDs, n=6) of 2.8%-7.5%. [Conclusions] This method is simple and accurate, and provides technical reference for food safety monitoring. 

 摘要： [Objectives] This study was conducted to improve the purification agent combination conditions for QuEChERS and establish a method for detecting the residues of 18 fungicides in animal-derived foods (fish, pork, milk, eggs, and pork liver) using high-performance liquid chromatography-triple quadrupole tandem mass spectrometry. [Methods] The samples were extracted with acetonitrile, purified with 885 mg of magnesium sulfate, 150 mg of PSA, and 15 mg of GCB, and analyzed using ACQUITY UPLC BEH C18 as the chromatographic column with a mobile phase of acetonitrile-0.1% formic acid aqueous solution. [Results] The 18 fungicides showed good linearity in the range of 5.00–200.00 μg/L, with correlation coefficients (R²) greater than 0.991. The limit of quantification (LOQ) was 0.01 mg/kg. The average recoveries ranged from 63.7% to 117.5%, and the relative standard deviations (RSDs) were between 0.22% and 6.33%. [Conclusions] This method is simple, rapid, and highly accurate, and provides technical reference for the detection and risk assessment of fungicides in animal-derived foods. 

 摘要： Fruit radish ‘Sheng Cui 745’, rich in anthocyanins, is a first-generation hybrid developed by crossing sterile line HSR2063A as the female parent and inbred line HSR1876 as the male parent. The plants exhibit moderate growth vigor, with a slightly flat leaf cluster. The leaves are dark green, pinnately lobed. There are approximately 14 leaves per plant, 30 cm in length. The fleshy roots are 18 cm in length and 8.5 cm in diameter, presenting a cylindrical shape with purple-red skin and flesh. The flesh is fine-textured, crisp, and slightly spicy. The fleshy roots have a anthocyanin content of 708.7 mg/L and exhibit excellent toughness. The yield of the variety can reach up to 56 700 kg/hm². It is suitable for open-field autumn cultivation in Shandong, Tianjin, Hebei, and other regions. 

 摘要： To further enhance the yield and quality of kiwifruit and promote the sustainable development of the kiwifruit industry, this paper summarized the characteristics, damage sites, and control methods of major kiwifruit diseases and pests. It pointed out the main issues in current kiwifruit pest and disease management and proposed corresponding solutions. The prevention and control of kiwifruit pests should adhere to the principle of "prevention first, integrated management", and standardized planting modes should be implemented. In this process, priority should be given to agricultural, physical, and biological control methods to effectively reduce the use of chemical pesticides.  

 摘要： Trichosanthis Fructus (Gualou) is the dried ripe fruit of Trichosanthes kirilowii Maxim. or Trichosanthes rosthornii Harms (Cucurbitaceae family). It demonstrates significant therapeutic effects in treating coronary heart disease and diabetes mellitus, while also possessing anti-cancer and anti-aging properties. Both Trichosanthis Pericarpium (Gualoupi) and Trichosanthis Semen (Gualouzi) are used medicinally. With the increasing clinical application of Trichosanthes medicinal materials and their preparations (such as Gualou Injection and Gualou Tablets), the demand for them is expected to grow substantially. To further investigate Trichosanthis Fructus, explore its medicinal effects and new applications, and promote the development of Trichosanthis Fructus-based products, this paper reviewed the chemical components of Trichosanthis Fructus. Furthermore, the differences in major chemical components among Trichosanthis Fructus, Trichosanthis Pericarpium and Trichosanthis Semen were compared, providing a scientific basis for establishing quality evaluation standards of Trichosanthes medicinal materials. 

 摘要： Soybeans are rich in protein, fats, vitamins, and minerals, serving as an important source of plant-based protein for humans. Summer soybean is widely cultivated in China, and improving its yield and quality is of great significance for ensuring food security and promoting agricultural economic development. This paper elaborated on the high-quality and high-yield cultivation techniques for summer soybean, including variety selection, seed treatment, field selection and land preparation, sowing techniques, field management, pest and disease control, and harvesting, aiming to provide scientific cultivation guidance for summer soybean growers to achieve both superior quality and high yield.

 摘要： [Objectives] This study was conducted to better understand the occurrence status of thrips in Central Hunan, clarify thrips species, and provide a basis for scientific control. [Methods] The occurrence of thrips in the Central Hunan Region were investigated and analyzed. Through field collection, molecular biological identification and sequencing, thrips populations in the region were identified. [Results] Six species of thrips infesting vegetable crops were identified in the Central Hunan Region, with Megalurothrips usitatus, Thrips palmi, and Frankliniella intonsa being the dominant species. These pests were particularly severe on leguminous and cucurbit crops. [Conclusions] Combined with integrated control strategies, this study provides theoretical and technical support for the scientific management of thrips in the Central Hunan Region, ensuring the sustainable development of the local vegetable industry.

 摘要： This study attempted to assess the lethal concentration (96-h LC50) effects of imidacloprid (neonicotinoid pesticide), thiamethoxam (neonicotinoid pesticide), and their combination on juvenile Zebrafish (Danio rerio). Each set of trials contained a control (de-chlorinated tap water), and the experiments were repeated three times. The fish (n=10) were randomly measured with an average length of (3.4 ± 0.34) cm and weight of (1 ± 0.1) g. The temperature was kept at 24 °C. Experiments 1 and 2 were designed to investigate at the acute toxicity of imidacloprid and thiamethoxam on juvenile zebrafish (Danio rerio) respectively, whereas experiment3 was aimed at the combined toxicity of IMI and THM on zebrafish. The tests followed the same study design, and each experiment used seven different logarithmic concentrations of imidacloprid insecticides (310.00, 317.08, 324.33, 331.74, 339.32, 347.07, 355.00 mg/L) and thiamethoxam (175.00, 185.52, 200.93, 215.30, 230.70, 247.20, 264.88 mg/L). The results show that THM is more toxic than IMI, with LC50 values of 190.34 mg/L for THM and 310.92 mg/L for IMI. Both individual toxicities showed a substantial positive connection (P < 0.05) with confidence limits of 321.50 - 300.68 mg/L for IMI and 199.91 - 181.21 mg/L for THM. The joint toxicity test was carried out using the 96-h LC50 values of imidacloprid and thiamethoxam obtained in the individual acute toxicity trials at a 1:1 ratio. The Additive Index (AI) demonstrated that imidacloprid and thiamethoxam acted synergistically on D. rerio. As a matter of fact, more research is needed to better understand the impact of IMI and THM on other aquatic organisms and also create strategies to mitigate its harmful effects on aquatic life.

 摘要： Facing the severe decline in beef cattle market prices and leveraging its status as a major cattle-raising city, Binzhou City has actively utilized the superior genetic resources of Bohai Black Cattle. The city has progressively transitioned from small-scale cattle farming to specialized and large-scale breeding communities by collaborating with experts from national and provincial industrial technology systems including the Chinese Academy of Agricultural Sciences, Shandong Academy of Agricultural Sciences, and Shandong Agricultural University. The breeding focus has shifted from Simmental crossbred cattle to purebred Bohai Black Cattle, hybrid cattle, Angus Cattle, and Hereford Cattle, driving the transformation and upgrading of the beef cattle industry. Emphasizing "germplasm source security", Binzhou has capitalized on the unique advantages of Bohai Black Cattle to achieve doubling in quantity, quality, and profitability of high-grade beef cattle breeds. This initiative supports ecological conservation and high-quality development in the Yellow River Basin. A Qilu Model will be established for national modern animal husbandry, further strengthening Shandong’s first beef market.