| 刊名 | Agricultural Biotechnology |
| 作者 | Jun LIANG1, Qing LI1, Xiong CHEN1, Lijun LI1, Zihao QU1, Yu WANG1, Xiaoling LI2, Shengfu YIN3 |
| 作者单位 | 1. GRG Metrology & TEST (Hunan) Co., Ltd., Changsha 410221, China; 2. Hunan Provincial Fisheries Research Institute, Changsha 410153, China; 3. Fishery Product Quality Inspection and Testing Center (Changsha) of the Ministry of Agriculture and Rural Affairs, Changsha 410153, China |
| DOI | DOI:10.19759/j.cnki.2164-4993.2025.03.003 |
| 年份 | 2025 |
| 刊期 | 3 |
| 页码 | 9-13,23 |
| 关键词 | QuEChERS; HPLC-MS/MS; Animal-derived; Fungicide |
| 摘要 | [Objectives] This study was conducted to improve the purification agent combination conditions for QuEChERS and establish a method for detecting the residues of 18 fungicides in animal-derived foods (fish, pork, milk, eggs, and pork liver) using high-performance liquid chromatography-triple quadrupole tandem mass spectrometry. [Methods] The samples were extracted with acetonitrile, purified with 885 mg of magnesium sulfate, 150 mg of PSA, and 15 mg of GCB, and analyzed using ACQUITY UPLC BEH C18 as the chromatographic column with a mobile phase of acetonitrile-0.1% formic acid aqueous solution. [Results] The 18 fungicides showed good linearity in the range of 5.00–200.00 μg/L, with correlation coefficients (R²) greater than 0.991. The limit of quantification (LOQ) was 0.01 mg/kg. The average recoveries ranged from 63.7% to 117.5%, and the relative standard deviations (RSDs) were between 0.22% and 6.33%. [Conclusions] This method is simple, rapid, and highly accurate, and provides technical reference for the detection and risk assessment of fungicides in animal-derived foods. |
