| 刊名 | Agricultural Biotechnology |
| 作者 | Agnes Adu-Gyamfi 1, Mingxiao LI 1, Shunlong MENG1, 2*, Xi Chen1 |
| 作者单位 | 1. Wuxi Fishery Collage, Nanjing Agricultural University, Wuxi 214081, China; 2. Scientific Observing and Experimental Station of Fishery Resource and Environment in the Lower Reaches of the Changjiang River, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081, China |
| DOI | DOI:10.19759/j.cnki.2164-4993.2025.03.009 |
| 年份 | 2025 |
| 刊期 | 3 |
| 页码 | 36-39 |
| 关键词 | Acute toxicity (LC50); Joint toxicity; Imidacloprid; Thiamethoxam; Zebrafish (Danio rerio) |
| 摘要 | This study attempted to assess the lethal concentration (96-h LC50) effects of imidacloprid (neonicotinoid pesticide), thiamethoxam (neonicotinoid pesticide), and their combination on juvenile Zebrafish (Danio rerio). Each set of trials contained a control (de-chlorinated tap water), and the experiments were repeated three times. The fish (n=10) were randomly measured with an average length of (3.4 ± 0.34) cm and weight of (1 ± 0.1) g. The temperature was kept at 24 °C. Experiments 1 and 2 were designed to investigate at the acute toxicity of imidacloprid and thiamethoxam on juvenile zebrafish (Danio rerio) respectively, whereas experiment3 was aimed at the combined toxicity of IMI and THM on zebrafish. The tests followed the same study design, and each experiment used seven different logarithmic concentrations of imidacloprid insecticides (310.00, 317.08, 324.33, 331.74, 339.32, 347.07, 355.00 mg/L) and thiamethoxam (175.00, 185.52, 200.93, 215.30, 230.70, 247.20, 264.88 mg/L). The results show that THM is more toxic than IMI, with LC50 values of 190.34 mg/L for THM and 310.92 mg/L for IMI. Both individual toxicities showed a substantial positive connection (P < 0.05) with confidence limits of 321.50 - 300.68 mg/L for IMI and 199.91 - 181.21 mg/L for THM. The joint toxicity test was carried out using the 96-h LC50 values of imidacloprid and thiamethoxam obtained in the individual acute toxicity trials at a 1:1 ratio. The Additive Index (AI) demonstrated that imidacloprid and thiamethoxam acted synergistically on D. rerio. As a matter of fact, more research is needed to better understand the impact of IMI and THM on other aquatic organisms and also create strategies to mitigate its harmful effects on aquatic life. |
