| 刊名 | Agricultural Biotechnology |
| 作者 | Lin TIAN1,2,3#, Jinlu LI1,2,3#, Yi ZHANG1,2,3#, Aili XIANG1,2,3, Baiqin ZHENG1,2,3, Zhe YIN1,2,3, Dan WU1,2,3*, Caihua ZHANG1,2,3*, Lei WANG1,2,3* |
| 作者单位 | 1. Tangshan Food and Drug Comprehensive Inspection and Testing Center, Tangshan 063000, China; 2. Hebei Agricultural Products Quality and Safety Testing Technology Innovation Center, Tangshan 063000, China; 3. Tangshan Institute of Industrial Technology for Functional Agricultural Products, Tangshan 063000, China |
| DOI | DOI:10.19759/j.cnki.2164-4993.2025.03.002 |
| 年份 | 2025 |
| 刊期 | 3 |
| 页码 | 6-8,16 |
| 关键词 | HPLC-MS/MS; Bean sprout; Plant growth regulators; Mass spectrometry |
| 摘要 | [Objectives] This study was conducted to rapidly qualitatively and quantitatively analyze the residues of 10 plant growth regulators (PGRs) in bean sprouts. [Methods] Using bean sprouts as the test material, a high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) method was established to determine the residual levels of 10 PGRs in bean sprouts. [Results] Under optimized conditions, the retention time of the 10 PGRs ranged from 6.45 to 11.43 min. When the mass concentration ranged from 0.005 to 0.05 μg/ml, all PGRs exhibited good linearity, with correlation coefficients (r)≥0.999 1. The limits of detection (LODs, S/N=3) were in the range of 0.30-0.92 μg/kg, and the limits of quantification (LOQs) were in the range of 0.50-2.10 μg/kg. The average recovery values at three concentration levels ranged from 80% to 105.8%, with relative standard deviations (RSDs, n=6) of 2.8%-7.5%. [Conclusions] This method is simple and accurate, and provides technical reference for food safety monitoring. |
