| 刊名 | Medicinal Plant |
| 作者 | Juandi WANG, Rujuan ZHU, Mingtong ZHANG, Xin LI, Xiaoxia GUO, Donghua LI, Xiao MA |
| 作者单位 | 1.Gansu Provincial Institute of Drug Control, Lanzhou 730070, China; 2.NMPA Key Laboratory for Quality Control of TCM, Lanzhou 730070, China; 3.Shandong Vocational Animal Science and Veterinary College, Weifang 261060, China |
| DOI | DOI:10.19600/j.cnki.issn2152-3924.2026.02.006 |
| 年份 | 2026 |
| 刊期 | 2 |
| 页码 | 25-28 |
| 关键词 | High-performance liquid chromatography (HPLC), Xanthopurpurin, Rubia cordifolia, Charred R. cordifolia, Processing temperature, Processed product, Content determination, Quality evaluation |
| 摘要 | [Objectives] To establish a high-performance liquid chromatography (HPLC) method for comparing the content differences of xanthopurpurin in raw Rubia cordifolia and its charred products processed at different temperatures, sourced from various regions. [Methods] The chromatographic separation was performed using an HPLC COLUMN C18. The mobile phase consisted of acetonitrile-0.2% phosphoric acid solution (50:50, v/v) at a flow rate of 1.0 mL/min. Detection was carried out at 245 nm, and the column temperature was maintained at 30 ℃. [Results] Xanthopurpurin exhibited a good linear relationship within the range of 6.712～83.905 μg/mL (r=1.000 0). The average recovery rate was 101.3% with an RSD of 2.1% (n=6). Content determination results showed that the xanthopurpurin content in raw R. cordifolia ranged from 0.040 0 to 0.169 3 mg/g. In charred R. cordifolia processed at 110-130 ℃, it ranged from 0.309 2 to 0.380 1 mg/g, while in charred R. cordifolia processed at 350-370 ℃, it ranged from 0.512 8 to 1.168 8 mg/g. A significant increasing trend in xanthopurpurin content was observed with rising charring temperatures. [Conclusions] This method is simple, accurate, feasible, and reproducible. It can effectively distinguish between raw R. cordifolia and its charred products processed at different temperatures, providing a scientific basis for the quality evaluation of charred R. cordifolia and its formulations in traditional Chinese medicine. |
