| 刊名 | Asian Agricultural Research |
| 作者 | Fang LI， Sitong QUAN， Yaodong QIU |
| 作者单位 | Dandong Ande Biotechnology Co., Ltd.； College of Marine Science and Environment, Dalian Ocean University |
| DOI | 10.19601/j.cnki.issn1943-9903.2025.12.004 |
| 年份 | 2025 |
| 刊期 | 12 |
| 页码 | 13-17 |
| 关键词 | High Performance Liquid Chromatography-Tandem Mass Spectrometry (HPLC-MS/MS), Perfluoroalkyl and polyfluoroalkyl substances (PFAS), Aquatic products, QuEChERS, Limit of detection (LOD) |
| 摘要 | [Objectives] To extract and purify perfluoroalkyl and polyfluoroalkyl substances (PFAS) from aquatic products and determine the optimal pretreatment conditions. [Methods] The QuEChERS method was employed for the extraction and purification of PFAS in aquatic products. The detection of PFAS was carried out using liquid chromatography-tandem mass spectrometry (LC-MS/MS) in ESI negative ion mode with multiple reaction monitoring (MRM), and quantification was performed using the external standard method. By optimizing instrument parameters such as ion source temperature, spray voltage, and collision energy, high selectivity and sensitivity of the detection were ensured. [Results] The established calibration curves for 28 PFAS compounds compounds, covering a range of 0.02 to 20 ng/mL, demonstrated excellent linearity. Spike recovery tests yielded limits of detection (LOD) between 0.02 and 0.5 μg/kg for the 28 PFAS compounds compounds, with recovery rates ranging from 72.2% to 113.0%. [Conclusions] The method established in this study demonstrates excellent linearity, and is accurate, reliable, efficient, simple, and rapid. It possesses considerable practical applicability and can be used for the quality and risk assessment of 28 PFAS compounds compounds in aquatic products, indicating high practical utility. |
