| 刊名 | Asian Agricultural Research |
| 作者 | Hong YUE， Shuo TANG， Jianying LIANG， Xiaoyan HUANG， Wenhui HU， Lijun LIU， Xiaoli WU， Xue HU |
| 作者单位 | Inner Mongolia Yili Industrial Group Co.,Ltd.； Key Laboratory of Risk Prevention and Control Technology for Bovine,Ovine Milk and Meat Products,State Administration for Market Regulation |
| DOI | 10.19601/j.cnki.issn1943-9903.2025.10.008 |
| 年份 | 2025 |
| 刊期 | 10 |
| 页码 | 37-41 |
| 关键词 | Spectrophotometry,Total flavonoids,Yellow complex,Benzopyran ring,Rutin |
| 摘要 | [Objectives]Based on spectrophotometry,a method for determining the total flavonoid content in milk powder was established by optimizing sample pretreatment conditions,and method validation was performed.[Methods]Total flavonoids in milk powder were extracted with ethanol using 45 ℃ water bath ultrasonication for 60 min.Flavonoids contain a benzopyran ring structure and react with aluminum nitrate in a potassium acetate environment to form a yellow complex.The absorbance of this complex was measured at 420 nm for quantitative determination.[Results]The method exhibited a good linear relationship within the range of 0.2-1.0 mg.The limit of detection (LOD) was 0.05 g/100 g,and the limit of quantification (LOQ) was 0.3 g/100 g.When the spiked amount of total flavonoids was in the range of 0.3% to 1.0%,the spike recovery rates were 99.3% to 103.4%,and the relative standard deviations (RSDs) were less than 3.0%.[Conclusions]This method offers advantages such as rapidness,accuracy,good stability,and high sensitivity,and can be used for the detection of total flavonoid content in milk powder. |
