| 摘要 |
[Objectives] To develop a pair of specific primers for the PCR amplification of the full-length relA gene from Vibrio alginolyticus strain HY9901, as well as to conduct bioinformatics analysis. [Methods] The relA gene was amplified through PCR, and the resulting gene sequence was subsequently analyzed using bioinformatics tools, including amino acid sequence prediction, functional site analysis, subcellular localization prediction, and homology comparison. [Results] The relA gene had a total length of 2 220 bp and encoded 739 amino acid residues. The molecular weight was approximately 84.126 1 kDa, and its isoelectric point was 5.95. The protein lacked a signal peptide and transmembrane regions, while exhibiting multiple phosphorylation sites. Predictions regarding its subcellular localization suggested that it was predominantly situated in the cytoplasm. The amino acid sequence demonstrated a homology of 97% to 99% with other species within the genus Vibrio, and it clustered within the same subfamily as V. antiquarius and V. diabolicus. In the prediction of secondary structure, the proportions of α-helix, extended strand, random coil, and β-sheet were 54.13%, 12.04%, 28.15% and 5.68%, respectively. The similarity between the tertiary structure model and template 5kpw.1.w was 66%. [Conclusions] In this study, the relA gene of V. alginolyticus strain HY9901 has been successfully amplified and analyzed. The structural characteristics and potential functions of the encoded protein have been elucidated, thereby providing foundational data for understanding the role of this gene in V. alginolyticus. |
