 摘要： [Objectives] To explore biodiversity characteristics of Yuncheng Salt Lake and provide protection countermeasures. [Methods] According to the field survey data of 978 plant quadrats and cluster analysis, species diversity indicators such as Margalef richness index and Shannon-Wiener index were selected to deeply explore the structure and diversity characteristics of higher plant communities in Yuncheng Salt Lake. [Results] There were 117 species belonging to 97 genera and 35 families of higher plants in Yuncheng Salt Lake, of which Asteraceae and Gramineae were dominant families, and the North Temperate Distribution Genus accounted for 27.35% of the total genera; according to profile coefficient method, the optimal cluster number was 7, which was divided into 7 plant community types, including Artemisia capillaris community and Suaeda glauca (Bunge) community; the Lolium perenne community was the highest (d=2.537), the Artemisia scoparia community was the best (E=0.658), the Suaeda salsa (L.) Pall community was the lowest (H′=1.779), and the spatial distribution of species showed a significant aggregation pattern. [Conclusions] We put forward some countermeasures for biodiversity protection, including constructing dynamic monitoring system, implementing adaptive management strategy and promoting multi-party collaborative governance, so as to provide scientific theoretical basis and practical guidance for ecological restoration and sustainable development of Yuncheng Salt Lake.

 摘要： The implementation of the ecological protection red line serves as a crucial protective barrier for the conservation of wild plants with extremely small populations by delineating specific geographical areas designated for their conservation. The implementation of a comprehensive set of conservation measures aimed at establishing a biodiversity conservation network for wild plants with extremely small populations has laid the foundation for the health and stability of ecosystems, as well as the harmonious coexistence of humans and the natural environment. Research on the conservation of wild plants with extremely small populations serves as a critical foundation for informing and enhancing the effective conservation of such populations. This paper provides a systematic examination of the theoretical and practical conservation status of certain wild plants with extremely small populations and discusses the conservation trends of these populations within the framework of ecological protection red line delineation. In the future, strategies for the conservation of wild plants with extremely small populations should be integrated with an analysis of the causes of endangerment. This approach will provide a robust foundation for applied research focused on the conservation of these vulnerable plant populations.

 摘要： This article reviews the construction process of National Wetland Parks, the ecological effects of blue-green spaces, and their significance in wetland park development. It explores the functions of wetland parks in biodiversity conservation, water purification, climate regulation, and other ecological functions. Finally, it proposes of enhancing the protection and management of wetland parks, in the hope of promoting the full realization of their ecological benefits and ensure sustainable development.

 摘要： This study conducts a comprehensive and in-depth investigation into the current status of Southeast Asian international student cultivation at Pu’er University through multiple methods, including questionnaires, interviews, and literature research. The findings reveal that Pu’er University has achieved remarkable results in cultivating Southeast Asian international students, attracting a significant number of students from Southeast Asian countries while providing them with high-quality educational resources and a conducive learning environment. During their academic journey at Pu’er University, international students have not only acquired professional knowledge but also gained a profound understanding of Chinese culture and history, actively contributing to cultural exchanges and collaboration between China and Southeast Asia. Additionally, the study identifies existing challenges, including the need for improvement in curriculum design, faculty capacity, and cultural exchange initiatives. To address these issues, this paper proposes targeted strategies and recommendations, aiming to serve as a reference for Pu’er University and similar institutions in optimizing Southeast Asian international student cultivation.

 摘要： [Objectives] To develop a pair of specific primers for the PCR amplification of the full-length relA gene from Vibrio alginolyticus strain HY9901, as well as to conduct bioinformatics analysis. [Methods] The relA gene was amplified through PCR, and the resulting gene sequence was subsequently analyzed using bioinformatics tools, including amino acid sequence prediction, functional site analysis, subcellular localization prediction, and homology comparison. [Results] The relA gene had a total length of 2 220 bp and encoded 739 amino acid residues. The molecular weight was approximately 84.126 1 kDa, and its isoelectric point was 5.95. The protein lacked a signal peptide and transmembrane regions, while exhibiting multiple phosphorylation sites. Predictions regarding its subcellular localization suggested that it was predominantly situated in the cytoplasm. The amino acid sequence demonstrated a homology of 97% to 99% with other species within the genus Vibrio, and it clustered within the same subfamily as V. antiquarius and V. diabolicus. In the prediction of secondary structure, the proportions of α-helix, extended strand, random coil, and β-sheet were 54.13%, 12.04%, 28.15% and 5.68%, respectively. The similarity between the tertiary structure model and template 5kpw.1.w was 66%. [Conclusions] In this study, the relA gene of V. alginolyticus strain HY9901 has been successfully amplified and analyzed. The structural characteristics and potential functions of the encoded protein have been elucidated, thereby providing foundational data for understanding the role of this gene in V. alginolyticus.

 摘要： This paper provides a systematic review of Matrix-Assisted Laser Desorption/Ionization-Mass Spectrometry Imaging (MALDI-MSI), encompassing its technical principles, experimental workflows, matrix optimization strategies, and recent advancements in plant science applications. It highlights the method’s groundbreaking applications in spatial mapping of plant metabolites, dynamic hormone monitoring, and functional studies of tissue microdomains, while offering critical insights into current technical limitations and future research directions.

 摘要： With the rapid development of China’s economy and the government’s issuance of a series of rural revitalization strategies, the design of rural landscapes has garnered increasing attention. However, some rural landscape designs have become more urbanized, neglecting the natural ecology, distinctive characteristics, and cultural heritage of rural areas, leading to a homogenized phenomenon. To address issues such as landscape homogenization and cultural erosion in traditional villages, this paper analyzes the current status of ethnic minority village landscapes and integrates landscape design principles to propose design strategies for rural landscapes in traditional ethnic minority villages in Hunan Province. It is intended to better achieve the construction of rural landscapes in traditional ethnic minority villages in Hunan Province.

 摘要： The educational philosophies of curriculum-based ideological and political education and professional innovation-integrated education, both serving as guiding ideologies aligned with contemporary educational development, have been persistently deepened in the pedagogical practices of higher education institutions in recent years, significantly propelling the advancement of China’s higher education. However, research remains scarce regarding how these two approaches can mutually complement each other to jointly advance the education of the Acupuncture and Tuina Discipline. Consequently, this study explores pathways for innovation-integrated education in the Acupuncture and Tuina Discipline from the perspective of curriculum-based ideological and political education. Through educational research and practical implementation, it aims to provide innovative ideas and methods for enhancing the quality of talent cultivation in the Acupuncture and Tuina Discipline.

 摘要： A survey was conducted among 616 students from six private undergraduate universities in Hefei City, Anhui Province. Using Likert 5-point scale measurements and SPSS 27.0 for data analysis, the study revealed that students with prior entrepreneurial experience demonstrated lower satisfaction with university entrepreneurship education, and their past entrepreneurial attempts showed a negative correlation with future entrepreneurial intentions. However, high-quality entrepreneurship education exhibited a positive mediating role in this relationship. This study reveals the intrinsic mechanisms through which personal experiences and entrepreneurship education influence college students’ entrepreneurial intentions, providing theoretical references for higher education institutions to optimize entrepreneurship education programs and enhance students’ entrepreneurial motivations. These findings will contribute to the national strategy of "promoting employment through entrepreneurship".

 摘要： The disconnection between teaching, learning, and evaluation is particularly pronounced in traditional high school chemistry teaching. To align with the demands of the new curriculum standards for talent development, it is essential to implement reforms and innovations in teaching methods. This paper initially elucidates the integrated concept of teaching, learning, and evaluation, as well as its practical significance in the classroom. Subsequently, it explores the effective teaching design centered on the theme of iron and its compounds, actively investigating the implementation approach of the integration principle of teaching, learning, and evaluation in classroom. Furthermore, the paper emphasizes the pivotal role of the evaluation part in fostering the professional development of teachers and enhancing the core competencies of students, ultimately aiming to achieve high efficiency and quality in chemistry classroom teaching.