| 刊名 | Medicinal Plant |
| 作者 | Xue JIANG，Wenli LI，Yong CHEN，Jiabao MA |
| 作者单位 | The First Affiliated Hospital of Guangxi University of Chinese Medicine； Guangxi University of Chinese Medicine； The Second People’s Hospital of Qinzhou |
| DOI | 10.19601/j.cnki.issn2152-3924.2023.04.010 |
| 年份 | 2023 |
| 刊期 | 4 |
| 页码 | 38-40 |
| 关键词 | Toad skin, High performance liquid chromatography, Determination of content |
| 摘要 | [Objectives] To establish a method for the determination of active components in toad skin. [Methods] HPLC method was used to determine the content of five active components (bufotalin, cinobufotalin, bufalin, cinobufagin and resibufogenin) in toad skin. [Results] Chromatographic conditions are as follows: Agilent ZORBAX SB-C18 chromatographic column was used; acetonitrile (A)-0.3% glacial acetic acid (B) gradient elution (0-15 min, 28%A-54%A; 15-35 min, 54%A-54%A) was conducted; the flow rate was 0.6 mL/min; the detection wavelength was 296 nm; the column temperature was 30 ℃; the sample size was 10 μL. Under the above conditions, the determination method of the five components can be established at one time. [Conclusions] The method was stable and reliable, and can provide experimental basis for the development and utilization of active ingredients in toad skin. |
