| 摘要 |
[Objectives] To explore the inhibitory effect of octadecadienoic acid (ODA) on proliferation and apoptosis of glioma cells and its mechanism. [Methods] Cultured human glioma cells (cell density 2×106 cells/L) were divided into three groups: solvent control group (DMSO, 30 μL/L), 5-FU group (10 mg/L) and octadecadienoic acid group (0.3, 0.6, 1.2 mg/L). The toxic effects of ODA on glioma cells were detected by trypan blue and thiazolium blue (MTT). The expression of P53, PI3K, P21, PKB/Akt and caspase-9 protein in glioma cells were detected by enzyme-linked immunosorbent assay (ELISA). [Results] The cell count under optical microscope showed that the inhibition rate of cell proliferation in low, medium and high dose ODA groups and 5-FU group was significantly higher than that in solvent control group (P<0.01), but there was no significant difference compared with 5-FU group (P>0.05). The results of MTT showed that compared with the solvent control group, the inhibition rate of cell proliferation in low, medium and high dose ODA groups and 5-FU group significantly increased (P<0.01); compared with 5-FU group, the inhibition rate of cell proliferation in high dose ODA group significantly increased (P<0.01). The results of flow cytometry showed that compared with the solvent control group, the number of cells in G0/G1 phase increased significantly (P<0.05, P<0.01), the number of cells in G2/M phase decreased significantly (P<0.01) and the apoptosis rate increased significantly (P<0.01) in the low, medium and high dose ODA groups and 5-FU group; compared with 5-FU group, the number of cells in G2/M phase decreased significantly (P<0.01) and the apoptosis rate increased significantly (P<0.01) in ODA group. ELISA testing results showed that the expression levels of P53, P13K and PKB/Akt in low, medium and high dose ODA groups and 5-FU group were significantly lower than those in solvent control group (P<0.01), and only the expression level of protein in high dose ODA group was significantly lower than that in 5-FU group (P<0.01); the expression levels of P21 and caspase-9 in low, medium and high dose ODA groups and 5-FU group were significantly higher than those in solvent control group (P<0.05, P<0.01), but the expression level of protein in high dose ODA group was significantly higher than that in 5-FU group (P<0.01). [Conclusions] ODA can obviously inhibit the proliferation of glioma cells and induce apoptosis. The mechanism is related to up-regulation of P21, caspase-9, down-regulation of P53, PI3K, PKB/Akt, inhibition of cell division cycle and decrease of PI3K-Akt signal transduction pathway. |
