| 摘要 |
[Objectives] To investigate the effect and mechanism of Dachengqi Decoction and separated decoction on incomplete intestinal obstruction in rats. [Methods] 80 healthy SD rats were selected to establish incomplete intestinal obstruction model by silk ligation. The dosage was 20 mL/kg for 3 d, and the damage index of ileocecal mucosa was analyzed; the morphology of ileocecal mucosa was observed by HE staining; the serum levels of IL-1α, IL-1β, IL-6, IL-18, Ach, NO, ET, IL-1, TNF-α and ultra-micro Na+-K+-ATPase were detected by ELISA. [Results] Compared with the model group, the mucosal damage index of Dachengqi Decoction and each separated decoction group decreased significantly (P<0.05); compared with the normal group and sham operation group, the serum level of IL-1, IL-6, TNF-α and other factors in the model group increased significantly (P<0.05); compared with the model group, the serum IL-1, IL-6 and TNF-α secretion levels of rats in Dachengqi Decoction group and separated decoction group decreased (P<0.01). [Conclusions] Dachengqi Decoction and each separated decoction can effectively improve intestinal tissue pathological damage in the incomplete intestinal obstruction model rats, and reduce the inflammatory reaction in the rat body. |
