| 刊名 | Medicinal Plant |
| 作者 | Wenyu MA， Jincun LI， Xuemei PAN， Haoran NI， Yuhuan XIE |
| 作者单位 | School of Chinese Materia Medica, Yunnan University of Chinese Medicine； Yunnan Provincial University Key Laboratory of Aromatic Chinese Herb Research； Yunnan Innovation Team of Application Research on Traditional Chinese Medicine Theory of Disease Prevention at Yunnan University of TCM |
| DOI | 10.19601/j.cnki.issn2152-3924.2024.04.021 |
| 年份 | 2024 |
| 刊期 | 4 |
| 页码 | 92-98 |
| 关键词 | GC-MS/MS, Cang-ai volatile oil, MRM mode, Pharmacokinetics |
| 摘要 | [Objectives] To establish a gas chromatography-triple quadrupole mass spectrometry (GC-MS/MS) method based on multiple reaction monitoring (MRM) mode for the analysis of the major components in Cang-ai volatile oil (CAVO). [Methods] An ultrasensitive gas chromatography-tandem mass spectrometry (GC-MS/MS) method was developed and validated for the determination of three highly abundant components in rat plasma samples. Paeonol was used as an internal standard. A multiple reaction monitoring (MRM) model was employed for the quantification of the three major components of CAVO. [Results] The method demonstrated linearity over the range of 0.25 to 50 μg/mL with a correlation coefficient (R2) greater than 0.999 8. The lower limit of quantification was 0.25 μg/mL. Intra-day and inter-day accuracy and precision were within 15%. Extraction recovery and matrix effect values ranged from 90.1% to 110.6% and 0.1% to 2.1%, respectively. [Conclusions] This method was successfully applied to the simultaneous determination of the three components in high-level CAVO plasma samples, providing a basis for subsequent studies of CAVO. |
