| 摘要 |
[Objectives] To investigate the effects of quercetin extracted from flower buds of Sophora japonica cv. jinhuai on the proliferation, apoptosis and migration of human breast cancer MCF-7 cells. [Methods] MTT assay, inverted microscope observation, hoechst33342 staining, flow cytometry (FCM) and wound healing assay were adopted to investigate the proliferation, morphological changes, apoptosis level and cell migration ability of human breast cancer MCF-7 cells, respectively. [Results] The morphological changes of cells in the treatment groups included gradually decreased number, reduced volume, vague cell contour, loose intercellular connection, uneven cytoplasm distribution and increased cell debris. With the increase of drug concentration, quercetin significantly inhibited the proliferation of human breast cancer MCF-7 cells (P<0.05). The number of apoptotic bodies increased gradually. When the concentration reached 100 μmol/L, a large number of nuclear fragments appeared, and the level of apoptosis was statistically different (P<0.05). The mobility and migration ability of cells showed a decreasing trend, and the differences were statistically significant (P<0.05). [Conclusions] This study can provide experimental basis for clinical application of quercetin against breast cancer. |
