| 摘要 |
[Objectives] This study was conducted to investigate the effects of remifentanil pretreatment on inflammatory factors in rats with acute cerebral ischemia. [Methods] Sixty SD rats were randomly divided into the normal control group, sham operation group, ischemic brain injury group, and remifentanil pretreatment group. Except the normal control group, each group was divided into three subgroups (six in each group) according to the sampling time points of 6, 12 and 24 h after execution. After modeling, the rats were scored for neurological deficit, and observed for pathological changes of neurons in the brain tissue by HE staining and the brain infarct volume by TTC staining, and the expression levels of TNF-α, IL-6 and IL-8 were detected by RT-PCR. [Results] HE staining: No significant changes were observed in the pathological morphology of the brain tissue in the blank group and sham operation group; and the neuronal structure of rats in the acute cerebral ischemia group was obviously damaged, and the neuronal damage in the remifentanil pretreatment group was less than that in the acute cerebral ischemia group at each time point. TTC staining: The gray brain infarct area in the remifentanil pretreatment group was significantly smaller than that in the cerebral ischemia group (P<0.05). RT-PCR detection results: The expression levels of TNF-α, IL-6 and IL-8 in the blank group and sham surgery group did not show significant changes at different times (P>0.05); and compared with the cerebral ischemia group, the expression levels of TNF-α, IL-6, and IL-8 in the remifentanil pretreatment group were significantly reduced at all time points (P<0.05). [Conclusions] Remifentanil pretreatment could protect the brain by reducing the expression of inflammatory factors after cerebral ischemia injury. |
