| 摘要 |
[Objectives] To systematically screen the core active components, action targets, and signaling pathways of Qingxuan Zhiyang Foot Spray (Phellodendri Chinensis Cortex, Stemonae Radix, Sophorae Flavescentis Radix, Dictamni Cortex, Pseudolaricis Cortex, Kochiae Fructus, and Senecionis Scandentis Herba) based on network pharmacology, and to elucidate its molecular mechanism in the external treatment of tinea pedis and superficial skin fungal infections. [Methods] Potential active components and targets of the seven medicinal herbs were screened using the TCMSP database combined with physicochemical criteria for transdermal penetration of external preparations. Disease targets for tinea pedis, athlete’s foot, and superficial skin fungal infections were retrieved from the GeneCards database. Drug-disease overlapping targets were obtained using the Venny tool. A protein-protein interaction (PPI) network was constructed using the String database. A visualized “Chinese herbal medicine – active component – target” interaction network was constructed using Cytoscape 3.10.1, followed by topological analysis. GO functional enrichment and KEGG pathway enrichment were performed using the DAVID database, and the enrichment results were visualized using bubble plots and bar charts. [Results] A total of 132 active components, 418 drug targets, 452 disease targets, and 96 core overlapping targets were obtained. The PPI network revealed TNF, IL6, IL1B, AKT1, TP53, JUN, and PTGS2 as hub proteins. The core pharmacologically active substances were quercetin, luteolin, berberine, matrine, stemonine, and pseudolaric acid B. GO enrichment was mainly concentrated in processes such as inflammatory response, apoptosis, and keratinocyte proliferation. KEGG enrichment revealed that the PI3K-Akt, AGE-RAGE, MAPK, TNF, IL-17, and C-type lectin receptor signaling pathways were the core regulatory pathways. [Conclusions] Through the multi-component transdermal targeted action, this spray regulates targets and pathways related to inflammation, innate antifungal immunity, and skin barrier repair, thereby achieving the triple efficacy of bacteriostasis, anti-inflammatory and antipruritic effects, and barrier repair. This study reveals the molecular mechanism of “multi-component synergy and multi-pathway coordination” underlying the external treatment of superficial fungal infections with Chinese herbal compound formulas. |
