| 刊名 | Medicinal Plant |
| 作者 | Qiang MA, Xueyuan FENG, Xintong ZHAO, Hongwei LI, Yanjie LU, Yanzhen ZUO, Yuhong LI, Qian XU |
| 作者单位 | Institute of Basic Medicine, Chengde Medical University |
| DOI | DOI:10.19600/j.cnki.issn2152-3924.2026.04.019 |
| 年份 | 2026 |
| 刊期 | 4 |
| 页码 | 81-86 |
| 关键词 | Gastric cancer, RPS4X, Bioinformatics, RT-qPCR |
| 摘要 | [Objectives] To investigate the expression patterns of RPS4X in gastric cancer and the related molecular mechanisms promoting the progression of gastric cancer based on bioinformatics analysis, and identify potential targets for the early diagnosis and clinical management of gastric cancer. [Methods] Gene expression data for gastric cancer were obtained from the TCGA database. GEPIA2 and UALCAN were used to analyze differential expression of RPS4X and its relationship with patient survival prognosis. The protein-protein interaction (PPI) network was constructed using STRING. GO and KEGG enrichment analyses were performed using DAVID. GSEA was conducted with the clusterProfiler package in R language. The expression of RPS4X in normal gastric mucosal epithelial cells and gastric cancer cells was measured by RT-qPCR assay. [Results] The expression of RPS4X was significantly increased in gastric cancer tissues and correlated with poor patient prognosis (P<0.05). PPI network analysis indicated that RPS4X may interact with proteins such as RPL13, RPS23, RPS3, and RPS12. GO and KEGG enrichment analysis suggested that RPS4X may be involved in biological functions such as cytoskeletal remodeling and signaling pathways related to cornified envelope formation. These findings were further verified by GSEA. RT-qPCR results showed that RPS4X expression levels in gastric cancer cells were significantly higher than those in normal gastric mucosal epithelial cells (P<0.05). [Conclusions] RPS4X is highly expressed in gastric cancer and may promote the occurrence and progression of the disease by regulating biological processes such as cytoskeletal remodeling and related signaling pathways, and has the potential to serve as a target for the early diagnosis and treatment of gastric cancer. |
