| 摘要 |
[Objectives] To observe the protective effect of Danhong Decoction on cerebral ischemia‑reperfusion injury in Sprague-Dawley (SD) rats. [Methods] Eighteen SD rats were randomly divided into blank control group, MCAO group, and observation group (n=6 each). In the MCAO and observation groups, the cerebral ischemia‑reperfusion model was established by the suture occlusion method (ischemia for 60 min followed by reperfusion). After successful modeling, the observation group received 2% Danhong Decoction (2 mL/rat) by intragastric administration twice daily for 1 week, while the blank control and MCAO groups received an equal volume of normal saline. After treatment, the expression levels of endothelial nitric oxide synthase (eNOS), nitric oxide (NO), malondialdehyde (MDA), superoxide dismutase (SOD), vascular endothelial growth factor (VEGF), and endothelin‑B (ET‑B) receptor in brain tissue were measured. [Results] Compared with the MCAO group, the observation group showed significantly decreased levels of MDA, ET‑B protein, and ET‑B mRNA, and significantly increased levels of NO, eNOS, SOD, VEGF, and VEGF mRNA (all P < 0.05). [Conclusions] Danhong Decoction can attenuate cerebral ischemia-reperfusion injury in SD rats, and its mechanism may be related to inhibition of oxidative stress and promotion of angiogenesis. |
