| 刊名 | Medicinal Plant |
| 作者 | Binglan TANG, Wei GAO, Chengjian ZHAO, Chunli OU, Xiaoli HOU, Lu CHEN, Dandan MO |
| 作者单位 | National Traditional Chinese Medicine Inheritance and Innovation Center, Guangxi Botanical Garden of Medicinal Plant/Guangxi Key Laboratory of Medicinal Resource Protection and Genetic Improvement/Guangxi Zhuang Autonomous Region Traditional Chinese Medicine Resources Intelligent Creation Engineering Research Center, Nanning 530023, China; Guangxi Zhuang Autonomous Region Drug Administration, Nanning 530029, China |
| DOI | DOI:10.19600/j.cnki.issn2152-3924.2026.03.008 |
| 年份 | 2026 |
| 刊期 | 3 |
| 页码 | 30-35 |
| 关键词 | Yao medicine Kadsura longipedunculata, HPLC, Characteristic chromatogram, Quality control, Anwulignan, Anti-inflammatory action |
| 摘要 | [Objectives] To establish a characteristic chromatogram of the traditional Yao medicine Kadsura longipedunculata, develop a method for quantifying its primary component, anwulignan, and evaluate the anti-inflammatory activity of anwulignan. [Methods] Gradient elution was performed using high-performance liquid chromatography (HPLC) with a mobile phase consisting of acetonitrile and 0.5% phosphoric acid solution. A characteristic chromatogram of K. longipedunculata was established, and its similarity was assessed using the Similarity Evaluation System for Chromatographic Fingerprint of Traditional Chinese Medicine (2012 edition). Additionally, the content of anwulignan in K. longipedunculata was quantified. Lipopolysaccharide-induced RAW264.7 macrophages were employed as an inflammatory cell model to investigate the effects of anwulignan on the levels of tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), and interleukin-6 (IL-6) in the cell culture supernatant. [Results] The similarity indices of characteristic chromatograms for 10 batches of K. longipedunculata ranged from 0.901 to 0.994, with 9 common peaks identified. Among these, three components were characterized, including changnan schisantherin E, kadsulactone A, and anwulignan. The content of anwulignan was quantified as ranging from (0.72 ± 0.05) to (1.21 ± 0.03) mg/g (n = 3). Furthermore, anwulignan at concentrations of 0.125–0.5 μg/mL significantly reduced the levels of TNF-α, IL-1β, and IL-6 in the supernatant of inflammatory model cells (P < 0.05 or P < 0.01). [Conclusions] HPLC characteristic chromatogram of K. longipedunculata and the method for determining the content of anwulignan have been established. Anwulignan may represent the active ingredient responsible for the anti-inflammatory effects observed in K. longipedunculata. |
