| 刊名 | Medicinal Plant |
| 作者 | Jingyue ZHU, Jing LI,Yi OU, Dabiao JING, Chen HUANG, Yijie JIANG, Zhiqiang HUANG, Yufei ZHANG, Kaimei ZHU, Yulin HE |
| 作者单位 | The Second Affiliated Hospital of Guilin Medical University, Guilin 541199, China; Guilin Sanjin Pharmaceutical Co., Ltd., Guilin 541199, China; College of Pharmacy, Guilin Medical University, Guilin 54119, China |
| DOI | DOI:10.19600/j.cnki.issn2152-3924.2026.03.007 |
| 年份 | 2026 |
| 刊期 | 3 |
| 页码 | 26-29 |
| 关键词 | Ardisia gigantifolia extract, Pancreatic cancer, AsPC-1 cells, Oxidative stress, Cell apoptosis |
| 摘要 | [Objectives] To investigate the effects of Ardisia gigantifolia extract on oxidative stress and apoptosis in pancreatic cancer AsPC-1 cells. [Methods] MTT assay was used to detect the inhibitory effects of different concentrations (0, 2, 4, 6, 8, 10 μg/mL) of A. gigantifolia extract on the proliferation of AsPC-1 cells after 48 h treatment, and the half maximal inhibitory concentration (IC50) was calculated; Transwell assay was employed to evaluate the effects of the extract on cell migration and invasion; intracellular superoxide dismutase (SOD) activity and malondialdehyde (MDA) level were measured using commercial kits to reflect the oxidative stress status. [Results] A. gigantifolia extract significantly inhibited the proliferation of AsPC-1 cells (IC50 = 5.805 μg/mL), reduced their migration and invasion abilities, enhanced SOD activity, and decreased the oxidative stress level. [Conclusions] A. gigantifolia extract enhances SOD activity in pancreatic cancer AsPC-1 cells, reduces ROS levels, and induces apoptosis, thereby exerting an inhibitory effect on the viability of AsPC-1 cells. |
