| 刊名 | Medicinal Plant |
| 作者 | Jianli JIMU,Zina LI,Shaoshan ZHANG, Huachun SHENG,Yuan LIU,Wenbing LI |
| 作者单位 | School of Pharmacy and Food Science, Southwest Minzu University, Chengdu 610041, China; Sichuan Provincial Engineering Laboratory for Conservation and Utilization of Qiang-Yi Medicinal Resources, Chengdu 610225, China; Key Laboratory of National Ethnic Affairs Commission for Preservation and Utilization of Ethnomedicinal Resources in Qinghai-Tibet Plateau, Chengdu 610225, China |
| DOI | DOI:10.19600/j.cnki.issn2152-3924.2026.03.002 |
| 年份 | 2026 |
| 刊期 | 3 |
| 页码 | 6-10 |
| 关键词 | Nardostachys jatamansi, Gene expression level, Bioactive compound accumulation, Nardosinone, Evaluation method |
| 摘要 | [Objectives] To analyze the gene expression networks across distinct tissues of Nardostachys jatamansi and elucidate the correlation between key gene expression levels in leaves and nardosinone accumulation in medicinal rhizomes, providing a technical foundation for rapid monitoring of bioactive compound accumulation.[Methods] Transcriptome sequencing coupled with Weighted Gene Co-expression Network Analysis (WGCNA) was employed to construct gene co-expression networks, Ultra-performance Liquid Chromatography (UPLC) was utilized to quantify nardosinone content across various growth stages. Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR) was integrated to validate spatiotemporal relationships between key gene expression profiles and nardosinone accumulation patterns. [Results] Among seven co-expression modules, the blue module demonstrated an exceptionally strong correlation with nardosinone content (r=0.993, p=1.2×10⁻¹⁵). This module encompassed pivotal genes such as NjDXS3 and NjTPS22. Three-year-old N. jatamansi plants reached peak nardosinone accumulation in September, with leaf expression levels of NjDXS3 closely mirroring this accumulation trend, whereas NjTPS22 expression exhibited minimal temporal variation. A critical threshold ratio exceeding 4.5 for NjDXS3/NjTPS22 expression in leaves signaled peak nardosinone concentrations. [Conclusions] By monitoring the expression ratio of NjDXS3 to NjTPS22 in leaves, the accumulation status of bioactive compounds in medicinal organs can be non-invasively assessed, offering a novel methodology for quality evaluation of N. jatamansi crude drugs. |
