| 刊名 | Medicinal Plant |
| 作者 | Yizhang WANG, Jing LI, Jingyue ZHU, Kaimei ZHU, Peng XIE, Dabiao JING, Fujun CHEN, Shiqiang OU, Yulin HE, Shengjiu GU |
| 作者单位 | College of Pharmacy, Guilin Medical University, Guilin 541199, China; The Second Affiliated Hospital of Guilin Medical University, Guilin 541199, China;Guangxi Kelun Pharmaceutical Co., Ltd., Guilin 541805, China |
| DOI | DOI:10.19600/j.cnki.issn2152-3924.2026.03.001 |
| 年份 | 2026 |
| 刊期 | 3 |
| 页码 | 1-5，10 |
| 关键词 | Ceftazidime-avibactam (CAZ-AVI), Response surface methodology, Green analytical chemistry (GAC), Content determination, High performance liquid chromatography (HPLC) |
| 摘要 | [Objectives] To quantify the content of ceftazidime-avibactam (CAZ-AVI) employing green analytical chemistry principles and assess the environmental sustainability of the proposed method. [Methods] The concentrations of disodium hydrogen phosphate and potassium dihydrogen phosphate, as well as the pH of the buffer solution, were optimized using a Box-Behnken design. The evaluation method's performance was validated through methodological assessment, and its environmental sustainability was appraised employing the AGREE, BAGI, and Complex GAPI tools. [Results] The optimal conditions were determined to be 27.79 mmol/L disodium hydrogen phosphate, 20.27 mmol/L potassium dihydrogen phosphate, and a pH of 3.52. The linear ranges for ceftazidime (CAZ) and avibactam (AVI) were 0.09–2.25 and 0.1–3 μg/mL, respectively, with correlation coefficients exceeding 0.999. The respective contents were 87.61% and 9.90%. Recovery rates were 98.56% for CAZ and 97.57% for AVI, while the detection limits were as low as 0.44 and 0.75 ng/mL, respectively. The green chemistry evaluation yielded a favorable result, with an AGREE score of 0.85. [Conclusions] The method is sensitive, accurate, and environmentally sustainable, making it suitable for the quality inspection of CAZ-AVI. |
