| 刊名 | Medicinal Plant |
| 作者 | Haishen LUO, Shenggao YIN, Yanyan CHEN, Hanbai LIANG, Jiansen XU |
| 作者单位 | 1.Department of Pharmacy, The First Affiliated Hospital of Guangxi University of Chinese Medicine, Nanning 530023, China; 2.Guangxi University of Chinese Medicine, Nanning 530002, China; 3.Wuzhou Vocational College, Wuzhou 543003, China; 4.Department of Pharmacy, Guilin Municipal Hospital of Chinese Medicine, Guilin 541001, China |
| DOI | DOI:10.19600/j.cnki.issn2152-3924.2026.02.002 |
| 年份 | 2026 |
| 刊期 | 2 |
| 页码 | 8-11 |
| 关键词 | Antioxidant capacity，Bauhinia championii honey， DPPH， Radical scavenging activity |
| 摘要 | [Objectives]To investigate the impact of storage duration on the DPPH free radical scavenging activity in Bauhinia championii honey and to assess its antioxidant activity. [Methods] The free radical scavenging activity of 38 batches of B. championii honey (fresh honey stage, aged honey stage I, aged honey stage II), was evaluated using the microplate DPPH assay. Honey sample solutions at varying concentrations (10, 25, 40, 55, and 70 g/L) were prepared, and 100 μL of each was combined with 100 μL of 0.05 g/L DPPH ethanol solution. The reaction mixtures were incubated at room temperature in the dark for 30 min. Subsequently, absorbance was measured at 517 nm, and both the scavenging rate and the half maximal inhibitory concentration (IC50) were calculated. [Results] The IC50 values for the DPPH free radical scavenging activity in 38 batches of B. championii honey at the fresh honey stage ranged from 8.96 to 58.65 mg/mL, with a mean value of 30.48 mg/mL. During the aged honey stage I, the IC50 values ranged from 8.32 to 55.08 mg/mL, averaging 28.48 mg/mL. In the aged honey stage II, theIC50 values ranged from 16.71 to 68.01 mg/mL, with a mean of 41.91 mg/mL. The methodological evaluation revealed that the relative standard deviations (RSD) for precision, repeatability, and stability were 0.02%, 2.21%, and 2.23%, respectively. [Conclusions] The DPPH free radical scavenging activity of 38 batches of B. championii honey remained robust throughout a 3-year storage period. However, it is advisable to limit the storage duration to within 2 years during production and storage to better preserve its antioxidant properties. The IC50 value for DPPH free radical scavenging activity serves as a critical indicator for assessing the storage stability and freshness of B. championii honey. |
