| 摘要 |
[Objectives] To observe the effects of chloramphenicol prednisone liniment on anti-inflammatory and anti-pruritic responses and skin barrier function in an acute eczema mouse model and explore its potential underlying mechanism. [Methods] Twenty-four female SPF-grade ICR mice were randomly and equally assigned to three groups: the blank control group, the acute eczema group, and the chloramphenicol prednisone liniment group according to the random number table method, with 8 mice per group. Except for the blank control group, the acute eczema model was established by applying 2,4-dinitrochlorobenzene (DNCB) to the right dorsal area. On day 10 (d10), 0.1 mL of normal saline was administered to the modeling site in both the blank control group and the acute eczema group, whereas chloramphenicol prednisone liniment was applied to the positive drug group. Medication was applied twice daily in all three groups for a total duration of 14 d. Sixty minutes following the final administration of the drug, the development of eczema in mice was visually assessed, and the severity of skin lesions was scored. Trans-epidermal water loss (TEWL) was measured using a multifunctional skin tester. Experiments inducing and alleviating pruritus were performed to compare the frequency of mice licking their bodies, the latency period before pruritus onset, and the duration of pruritus episodes. Levels of histamine and substance P (SP) in the lesion tissues were quantified using enzyme-linked immunosorbent assay (ELISA). [Results] Compared to the acute eczema group, the chloramphenicol prednisone liniment group exhibited a prolonged latency period of pruritus, an increased inhibition rate, and a shortened duration of pruritus. Additionally, there was a significant reduction in the frequency of mice licking their bodies, as well as in six eczema severity indicators: redness and swelling, scratch marks, papules, blisters, exudation or erosion at the lesion site, and the degree of skin swelling. Furthermore, levels of TEWL, histamine, and SP were also significantly decreased (P<0.05). [Conclusions] Chloramphenicol prednisone liniment exhibits anti-inflammatory and anti-pruritic properties. Its mechanism of action may involve the inhibition of mast cell activation within the lesion tissues of eczema model mice, thereby reducing the release of histamine and other active substances. This process alleviates inflammatory damage associated with eczema and contributes to the restoration of skin barrier function. |
