| 摘要 |
[Objectives] To apply the novel induction heating (IH) technology for the extraction of Ophiopogon japonicus polysaccharide (OJPS), fully utilize the advantages of IH technology to improve the extraction yield of OJPS, and compare it with traditional Chinese medicine polysaccharide extraction methods. [Methods] Using the polysaccharide extraction yield as the evaluation index, the IH extraction process for OJPS was optimized through an L9(34) orthogonal test, and systematic methodological validation was conducted. Finally, the process parameters and procedure for extracting OJPS using IH technology were optimized. [Results] The optimal extraction process was as follows: solid-liquid ratio of 1：55, extraction time of 75 min, and alcohol precipitation ratio of 1：4. The methodological investigation showed that OJPS had a good linear relationship in the range of 20-140 μg/mL, with R2=0.999 3. The average recovery rate was 99.94% (RSD=1.32%). The RSDs for precision, repeatability, and stability were all less than 2%, indicating that the measurement method for OJPS extraction yield was excellent. The extraction yields of OJPS by reflux extraction, ultrasonic extraction, and IH extraction were 26.67%, 40.70%, and 51.74%, respectively. [Conclusions] The novel IH extraction technology is stable and reliable, has a significant impact on the extraction yield of OJPS, and also improves the conversion rate of OJPS. It is expected to become an emerging technology and research direction for the extraction of Chinese medicine polysaccharides. |
