| 摘要 |
[Objectives] To preliminarily investigate the morphological identification and content determination of mango seeds utilized in Tibetan medicine, thereby providing foundational data to support the further refinement of quality standards for mango seeds. [Methods] Powder microscopic examination, thin-layer chromatography (TLC), and high-performance liquid chromatography (HPLC) were employed to identify mango seeds sourced from various regions in Sichuan Province. In accordance with the 2020 edition of the Chinese Pharmacopoeia (Volume IV), the extract content, total ash, acid-insoluble ash, and moisture content of the mango seeds were quantitatively determined. [Results] The morphological and powder microscopic characteristics of mango seeds in Tibetan medicine were described in detail. The methanol extract was qualitatively identified using TLC, and the content of gallic acid in the medicinal samples was determined by HPLC. The total ash content of mango seeds ranged from 1.82% to 2.73%, while the acid-insoluble ash content varied between 0.08% and 0.55%. The extract content ranged from 12.16% to 24.06%, and the moisture content was between 6.75% and 8.98%. [Conclusions] Specifications for mango seeds in Tibetan medicine have been established, indicating that the total ash content should not exceed 4.0%, the acid-insoluble ash content should not exceed 2%, the content of dilute ethanol extract should be no less than 15.0%, the moisture content should not exceed 12.0%, and the gallic acid content should be at least 1%. These parameters serve as a foundation for the development of quality standards for mango seeds in Tibetan medicine. |
