| 摘要 |
[Objectives] Using Calamine Zinc Oxide Phytocomplex Cream as an example, this study employed network pharmacology to investigate the therapeutic potential and mechanism of action of the combination of calamine, zinc oxide, and plant extracts in eczema intervention. [Methods] Active constituents of Calamine Zinc Oxide Phytocomplex Cream were identified through screening using the HIT2.0, HERB, and TCMSP databases. Corresponding targets of the active constituents were predicted using NetInfer. The collected targets were intersected with eczema and atopic dermatitis (AD)-related targets obtained from the GeneCards database to identify the effective therapeutic targets of Calamine Zinc Oxide Phytocomplex Cream. The network diagram of effective active constituents versus therapeutic targets for Calamine Zinc Oxide Phytocomplex Cream was constructed and subjected to topological analysis using Cytoscape software. The Protein-Protein Interaction (PPI) network was established and analyzed using the String database, Cytoscape software, and the cytoHubba plugin to identify key hub genes. Gene Ontology (GO) enrichment analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis were performed on the therapeutic targets using the DAVID database. [Results] Screening identified 57 active constituents in Calamine Zinc Oxide Phytocomplex Cream, corresponding to 601 potential targets. Subsequent analysis revealed 72 core therapeutic targets of Calamine Zinc Oxide Phytocomplex Cream specifically relevant to eczema and AD. Analysis of the network diagram suggested that Calamine Zinc Oxide Phytocomplex Cream may exert anti-inflammatory and immunomodulatory effects through active constituents such as quercetin, luteolin, and apigenin, while concurrently repairing skin barrier function by acting on targets including AKT1, NF-κB, and STAT3. Furthermore, the inclusion of mineral-based medicines provides additional functions such as itch relief and reinforcement of the skin barrier. [Conclusions] Calamine Zinc Oxide Phytocomplex Cream combines organic and inorganic constituents, synergistically alleviating the adverse symptoms of eczema and AD through multiple pathways. |
