| 摘要 |
[Objectives] To explore the effects of Radix Salviae Miltiorrhizae water extract and Semen Ziziphi Spinosae compound on the β-secretase activity in mice exposed to aluminum maltolate. [Methods] A total of 60 healthy, clean-grade SPF mice were randomly assigned to four groups based on their body weight, with each group consisting of 15 mice. The groups included a control group, a model group, treatment group 1, and treatment group 2. The control group received an equivalent dose of normal saline, while the model group and treatment groups 1 and 2 were intraperitoneally injected with 0.3 mg/kg of aluminum maltolate solution for 60 d. Additionally, treatment groups 1 and 2 were injected with 0.3 mg/kg of Radix Salviae Miltiorrhizae water extract and 0.3 mg/kg of Semen Ziziphi Spinosae compound, respectively, starting from the 31st day for a total of 30 d. The cognitive functions of mice, specifically their learning and memory capabilities, were assessed using the Y-shaped water maze test at three distinct time points: prior to, during, and following the experimental procedure. Serum samples were collected for the analysis of various biochemical markers, including hemoglobin (Hb), total cholesterol (TC), triglycerides (TG), total protein (TP), alanine aminotransferase (ALT), and blood urea nitrogen (BUN). Additionally, brain tissues were harvested to evaluate the levels of glutathione peroxidase (GSH-PX) and acetylcholinesterase (AChE) in both serum and brain samples. The expression levels of α-secretase, β-secretase, and γ-secretase in mouse serum were quantified using enzyme-linked immunosorbent assay (ELISA). [Results] According to the final results of the Y-shaped water maze test, the administration of therapeutic drugs to mice resulted in a gradual reduction in both the swimming time and the distance traveled to reach the platform in treatment groups 1 and 2. Additionally, the number of errors made by these treatment groups was significantly greater than that observed in the control group, with statistically significant differences (P<0.01). Among the three groups subjected to subchronic aluminum exposure, statistically significant differences were observed in the levels of Hb, TC, TG, TP, ALT, BUN, brain GSH-PX, and brain AChE (P<0.05). Furthermore, with the increasing duration of therapeutic drug administration, the levels of β-secretase in the brains of mice in both the treatment groups and the model group exhibited a significant decrease, while the levels of α-secretase showed a significant increase. Additionally, the differences were statistically significant when compared to the control group (P<0.05). [Conclusions] Radix Salviae Miltiorrhizae water extract and Semen Ziziphi Spinosae compound will decrease the expression level of β-secretase activity in mice exposed to aluminum maltolate. |
