| 摘要 |
[Objectives] To investigate the effects of Zuogui Pill on peripheral blood cells, sex hormone levels, interleukin 1β (IL-1β) levels, ovarian follicle number and spleen index in SD rats damaged by 60Coγ rays. [Methods] Fifty 8-week-old female SPF SD rats were selected, 10 of which were in the normal group and were fed routinely without irradiation; the other 40 rats were irradiated with 60Coγ rays (6.0 Gy, LD40) for 24 h, and then divided into radiation model group, Progynova group, high and low dose of Zuogui Pill groups. Progynova group was treated with 0.09 mg Progynova; high dose Zuogui Pill group was treated with 4.725 g Zuogui Pill crude drug and low dose Zuogui Pill group was treated with 0. 945 g Zuogui Pill crude drug; radiation group was treated with 2 mL normal saline by gavage once a day for 21 d. The changes of peripheral blood cells in different time periods were detected; the follicle stimulating hormone (FSH) and estradiol (E2) in peripheral blood serum were detected by enzyme linked immunosorbent assay (ELISA). The expression levels of E2, luteinizing hormone (LH) and interleukin 1β (IL-1β) were detected, and the ovarian follicle number and spleen index were measured. [Results] After irradiation, the number of peripheral blood cells decreased, especially the number of white blood cells (P<0.05), the content of hemoglobin increased (P<0.05), the level of E2 decreased, the number of mature follicles decreased, the spleen index decreased, and the expression level of IL-1β increased. After the intervention of Zuogui Pill, the serum E2 level, number of mature follicles and spleen index increased, while the serum IL-1β, FSH and LH levels decreased, especially in the high dose Zuogui Pill group (P<0.05). [Conclusions] Zuogui Pill can promote the repair of ovarian function in rats with radiation injury, which may be related to the promotion of bone marrow hematopoiesis and the improvement of immune function, reflecting the theory of "kidney governing blood vessels". |
