| 作者 |
Hao ZHANG, Jianguo WANG, Xuejun ZHANG, Jihao CHEN, Qigan LIANG, Xiaofa FU, Min WANG, Bo ZHOU, Qiang GAO, Haibo WU, Yuan HUANG |
| 摘要 |
[Objectives] To investigate the chemical composition, antioxidant property, and nematicidal activity of Cucumis metuliferus and establish a theoretical foundation for the development of botanical nematicides. [Methods] The compounds were isolated and purified using silica gel, reversed-phase medium, low-pressure gel Sephadex LH-20 column chromatography, and preparative high-performance liquid chromatography. Their identification was based on physicochemical properties, various spectral analyses, and data reported in the literature. Subsequently, the nematicidal and antioxidant activities of the isolated compounds were evaluated. [Results] A total of four compounds were isolated from C. metuliferus, which were identified as isovitexin-2’’-O-glucoside (compound 1), 9E,11E-(13-oxo) tridecadienoic acid (compound 2), 2E,4E-tridecadienedioic acid (compound 3), and 9E,11E-(13-hydroxy) tridecadienoic acid (compound 4). Compound 1 demonstrated a notable degree of antioxidant activity, achieving a DPPH scavenging rate of 49.40% at a mass concentration of 1 mg/mL. Additionally, compounds 1, 3, and 4 exhibited varying levels of nematicidal activity. Specifically, compound 1 displayed strong nematicidal activity at a concentration of 2.0 mg/mL, resulting in a mortality rate of 90.5% against nematodes. Furthermore, compounds 3 and 4 achieved a mortality rate of 100% against nematodes at a concentration of 0.25 mg/mL. [Conclusions] This study reports the first isolation of four compounds: isovitexin-2’’-O-glucoside, 9E,11E-(13-oxo) tridecadienoic acid, 2E,4E-tridecadienedioic acid, and 9E,11E-(13-hydroxy) tridecadienoic acid from C. metuliferus. Notably, 2E,4E-tridecadienedioic acid and 9E,11E-(13-oxo) tridecadienoic acid are identified as novel natural products. Among these compounds, isovitexin-2’’-O-glucoside, 9E,11E-(13-oxo) tridecadienoic acid, and 9E,11E-(13-hydroxy) tridecadienoic acid exhibit nematicidal activity, whereas isovitexin-2’’-O-glucoside demonstrates antioxidant properties. |
