| 摘要 |
[Objectives] To explore the effects of Qishi Shengjiang Guiyuan Granules on inflammatory response and T lymphocyte subsets in peripheral blood of rats with idiopathic pulmonary fibrosis (IFP) associated with gastroesophageal reflux disease (GERD). [Methods] Twenty-four SPF SD rats were randomly divided into control group, model group, Chinese medicine group and western medicine group, with 6 rats in each group. Except the control group, the other three groups were used to establish the rat model of GERD combined with IPF by injecting hydrochloric acid into the lower end of esophagus and inhaling diluted bleomycin (5 mg/kg). Rats in the Chinese medicine group (14 g/kg), rats in the western medicine group (4.17 g/kg), rats in the control group and the model group were given the same volume of saline by gavage for 14 d. Morphological and pathological changes of esophageal and lung tissues were observed under light microscope, and T lymphocyte subsets (CD3+, CD4+, CD8+) and the ratio of CD4+/CD8+ in peripheral blood were detected by flow cytometry. [Results] Compared with the control group, the pulmonary tissue of the model group showed that the pulmonary interstitium was obviously thickened, the alveoli were mutually fused, the structure was obviously destroyed, the original alveolar structure was disappeared, the inflammatory cell infiltration was around the pulmonary capillaries and the alveolar space, and the basal cell hyperplasia and inflammatory cell infiltration were at the lower end of the esophagus. Compared with the model group, the degree of inflammatory cell infiltration and lung tissue damage in the Chinese medicine group and the western medicine group was significantly reduced, the inflammatory infiltration in the lower esophagus was significantly reduced, and the cell proliferation was reduced. Compared with the control group, the CD3+, CD4+, CD8+ , CD4+/CD8+ in the peripheral blood of the rats in the model group, the Chinese medicine group and the western medicine group decreased (P < 0.01). Compared with the model group, CD3+, CD4+, and CD4+/CD8+ increased (P > 0.05, P > 0.05, P < 0.01),CD8+ decreased (P > 0.05). Compared with the Chinese medicine group, CD3+, CD4+, and CD4+/CD8+ increased (P > 0.05, P > 0.05, P < 0.01) and CD8+ decreased (P > 0.05) in the western medicine group. [Conclusions] Qishi Shengjiang Guiyuan Granules can effectively improve the inflammation of the lower esophagus and lung tissues of the pulmonary fibrosis rats with GERD and IFP, and regulate the number of T lymphocyte subsets CD3+,CD4+, CD8+ cells and CD4+/CD8+ratio in peripheral blood. |
