| 刊名 | Medicinal Plant |
| 作者 | Junjie CAI， Fuhong LI， Tianyu WANG， Zhuorui HE， Kaiyue LI， Yufan ZANG， Liqun REN |
| 作者单位 | Hebei Key Laboratory of Nerve Injury and Repair,Chengde Medical University |
| DOI | 10.19601/j.cnki.issn2152-3924.2024.06.017 |
| 年份 | 2024 |
| 刊期 | 6 |
| 页码 | 75-78 |
| 关键词 | Alzheimer’s disease (AD),Human umbilical cord mesenchymal stem cells (hUC-MSCs),APP/PS1 mice,NLRP3 inflammasome |
| 摘要 | [Objectives]To investigate the effect of human umbilical cord mesenchymal stem cells (hUC-MSCs) on the NOD-like receptor protein 3 (NLRP3)/cysteinyl aspartate specific proteinase (Caspase-1) pathway within the cerebral cortex of a mouse model of Alzheimer’s disease (AD).[Methods]Twelve 6-month-old female APP/PS1 mice were randomly assigned to two groups:the model group (MOD,n=6) and the hUC-MSCs treatment group (MSC,n=6).Six 6-month-old C57BL/6N mice were utilized as a control group (CON,n=6).All mice underwent caudal vein injections of hUC-MSCs.Following a 4-week treatment,the mice from each group were euthanized.The expression levels of NLRP3,Caspase-1 protein,and mRNA in the cerebral cortex of each group were assessed using Western blotting and real-time fluorescence quantitative PCR assays.[Results]The results of immunoblotting showed that the expression levels of NLRP3 and Caspase-1 proteins in the MOD group were significantly higher than those observed in the CON group.Furthermore,the expression levels of NLRP3 and Caspase-1 proteins in the MSC group were found to be lower than those in the MOD group.Additionally,the findings from real-time fluorescence quantitative PCR assay demonstrated that the mRNA levels of NLRP3 and Caspase-1 in the MOD group were elevated compared to the CON group.Conversely,the mRNA levels of NLRP3 and Caspase-1 in the MSC group were reduced in comparison to the MOD group.[Conclusions]hUC-MSCs have the capacity to modulate the expression of the NLRP3/Caspase-1 pathway within the cerebral cortex of APP/PS1 mice.This modulation may be associated with the neuroinflammatory processes mediated by hUC-MSCs in the brains of APP/PS1 mice. |
