| 刊名 | Agricultural Biotechnology |
| 作者 | Liyan LI1*, Jiahui SHI2, Yixin ZHANG2, Binbin CHEN2, Zhengyang SONG3, Xinhui ZHANG1, Bingyue LI1 |
| 作者单位 | 1. Medical School, Huanghe Science & Technology University, Zhengzhou 450063, China; 2. Pharmacy College, Henan University, Kaifeng 475001, China; 3. Life Science College, Henan University, Kaifeng 475001, China |
| DOI | DOI:10.19759/j.cnki.2164-4993.2026.03.012 |
| 年份 | 2026 |
| 刊期 | 3 |
| 页码 | 62-66,77 |
| 关键词 | Neohesperidin; Nanoliposomes; UVB; Anti-photodamage |
| 摘要 | The present research was aimed to evaluate the protective effects of neohesperidin-loaded nanoliposomes (NH-NL) against UVB-induced photodamage in HaCaT cells. NH-NL were prepared by the thin-film ultrasonication method. UVB-irradiated HaCaT cells were treated with different concentrations of free NH or NH-NL. Cell viability, apoptosis rate, and senescence (SA-β-Gal staining) were assessed. NH exhibited intrinsic UVB absorption (250-400 nm). Free NH moderately increased cell viability in a dose-dependent manner, whereas NH-Liposomes markedly improved viability from 19.74% (UVB alone) to 63.90% at 0.2 mg/ml. NH-NL also dose-dependently reduced the UVB-induced apoptosis rate from 31.52% to 15.83% and decreased the number of senescent (SA-β-Gal-positive) cells. The results suggest that NH-NL significantly protect HaCaT cells from UVB radiation-induced cell death, apoptosis, and senescence, demonstrating strong potential as an anti-photoaging nanocarrier system. |
