Inhibitory Effect of Dehydrocavidine on the Proliferation, Migration, and Invasion of Liver Cancer Cells by Regulating Macrophage Differentiation
| 刊名 |
Agricultural Biotechnology |
| 作者 |
Yifeng MO1, Chun LI1, Zimeng HUANG2, Fan YANG2, Shanshan CHU2, Yongwen LI3* |
| 作者单位 |
1. College of Pharmacy, Guilin Medical University, Guilin 530200, China; 2. College of Clinical Medicine, Guilin Medical University, Guilin 530200, China; 3. Department of Pharmacology, College of Pharmacy, Guilin Medical University, Guilin 541199, China |
| DOI |
DOI:10.19759/j.cnki.2164-4993.2026.03.011 |
| 年份 |
2026 |
| 刊期 |
3 |
| 页码 |
55-61 |
| 关键词 |
Liver cancer; Dehydrocavidine; Tumor-associated macrophages; Migration; Invasion; Proliferation |
| 摘要 |
[Objectives] This study was conducted to investigate the effect of dehydrocavidine (DC) on macrophage differentiation and the effect of DC-treated macrophage conditioned medium on the malignant progression of human liver cancer cell lines SK-HEP-1 and Huh-7. [Methods] THP-1 cells were induced using PMA and differentiated into M0 macrophages, which were then further polarized into M2-like macrophages by induction with interleukin-4 (IL-4) and interleukin-13 (IL-13), while being treated with different concentrations of DC (5, 10, 20 μM). Cell viability was assessed by the CCK-8 assay. The expression of M2 macrophage-related markers and factors was detected by Western blot and real-time quantitative PCR. The conditioned media from macrophages in each group were collected and co-cultured with SK-HEP-1 and Huh-7 liver cancer cells. The migration, invasion, and proliferation abilities of the liver cancer cells were evaluated by the wound healing assays, Transwell invasion assays, and colony formation assays. Additionally, the expression changes of epithelial-mesenchymal transition (EMT)-related proteins were detected. [Results] The CCK-8 assay showed that DC had no significant cytotoxicity against THP-1-derived macrophages within the experimental concentration range. Compared with the model group, DC significantly downregulated the mRNA and protein expression levels of the M2 macrophage markers CD163 and CD206 as well as related factors, thereby inhibiting M2-like polarization of macrophages. Co-culture with conditioned medium from DC-treated macrophages significantly suppressed the migration, invasion, and colony formation abilities of SK-HEP-1 and Huh-7 cells and downregulated the expression of EMT-related proteins such as N-cadherin, Vimentin, Snail, and MMP9. [Conclusions] DC can inhibit the polarization of THP-1-derived macrophages toward the M2-like phenotype, thereby weakening their pro-tumor effects and subsequently suppressing the migration, invasion, and proliferation of hepatocellular carcinoma cells. |
