| 刊名 | Agricultural Biotechnology |
| 作者 | Lin WANG1*, Jiaqing ZHOU1, Qing LI1, Lingquan YE2, Qinghua YANG1, Xiujuan WU1, Feng LI1 |
| 作者单位 | 1.China Inspection and Certification Group Hunan Co., Ltd, Changsha 410021, China; 2. Steadychem (Shanghai) Co., Ltd., Shanghai 201314, China |
| DOI | DOI:10.19759/j.cnki.2164-4993.2026.03.008 |
| 年份 | 2026 |
| 刊期 | 3 |
| 页码 | 29-42,61 |
| 关键词 | GC-MS/MS; Matrix effect; Pesticide residues; Fruits and vegetables |
| 摘要 | [Objectives] This study was conducted to address the fragmentation of existing research on matrix effects in pesticide residue detection by gas chromatography-tandem mass spectrometry (GC-MS/MS) and to overcome the limitations of the single matrix single pesticide research model. [Methods] A comprehensive matrix effect evaluation matrix covering “88 pesticides × 19 fruit and vegetable matrices” was constructed for the first time. The interference patterns of fruit and vegetable matrices on pesticide residue detection were systematically investigated, and representative matrices were screened for each matrix effect gradient. [Results] Among the tested pesticides, 95.8% exhibited matrix enhancement effects (ME > 100%), while 4.2% showed matrix suppression effects. Among these, medium matrix enhancement effects in the range of 120%-150% accounted for the highest proportion, reaching 54.9%. Six pesticides including paclobutrazol and terbufos showed significant matrix enhancement effects in 18 matrices except bean sprout, while deltamethrin, tetrachlorvinphos, and edifenphos exhibited matrix suppression characteristics in most matrices. Meantime, bean sprout, cucumber, mango, carrot, and cowpea can serve as typical representative matrices for the relatively weak, weak, medium, strong, and very strong matrix enhancement intervals, respectively. [Conclusions] This study has clarified the matrix effect characteristics in a multi pesticide, multi fruit/vegetable system, providing a theoretical basis and technical support for high throughput and accurate detection of pesticide residues in fruits and vegetables. It holds significant application value for food safety risk monitoring and regulatory work. |
