| 刊名 | Agricultural Biotechnology |
| 作者 | Weike YANG1, Changxiong HU1, Qiongyan LI1, Zenghu LIU1, Fenfen TANG2* |
| 作者单位 | 1.Sericulture and Apiculture Research Institute, Yunnan Academy of Agricultural Sciences, Mengzi 661101, China; 2. School of Agronomy, Honghe University, Mengzi 661101, China |
| DOI | DOI:10.19759/j.cnki.2164-4993.2026.01.001 |
| 年份 | 2026 |
| 刊期 | 1 |
| 页码 | 1-4 |
| 关键词 | Bombyx mori; β-Fructofuranosidase; BmSuc1; BmSuc2; Gene expression |
| 摘要 | [Objectives] The present study was conducted to investigate the change rule of β-fructofuranosidase gene expression and its enzyme activity in the midgut of 5th instar silkworm (Bombyx mori), in order to provide a reference for illustrating the enzymatic mechanism of using β-fructofuranosidase to absorb sucrose nutrition from mulberry leaves. [Methods] Real-time fluorescent quantitative PCR was applied to analyze the expression of BmSuc1 and BmSuc2 in midgut of 5th-instar silkworm larvae, meanwhile the activities of β-fructofuranosidase was determined. [Results] BmSuc1 was expressed in the midgut of 5th-instar silkworm larvae at different developmental stages. Its expression was upregulated at the beginning of the 5th instar and during the peak feeding period, whereas BmSuc2 expression remained very low throughout the entire 5th instar. The activity of β-fructofuranosidase was relatively high during the peak feeding period of 5th-instar larvae, showing a trend of increasing first and then decreasing. [Conclusions] The expression pattern of the BmSuc1 gene and the changes in β-fructofuranosidase activity were generally consistent with the physiological process of sugar nutrient absorption and utilization from mulberry leaves in 5th-instar silkworms. It suggests that BmSuc1, as a sucrose hydrolase gene, plays a major role in the digestion and absorption of sucrose nutrients from mulberry leaves in the midgut tissue. |
