| 刊名 | Agricultural Biotechnology |
| 作者 | Shaobin LIU1,2,3,4, Shuyu QI1,2,3,4,Kun ZHAI2,4*, Zengbao WU1,2,3,4* |
| 作者单位 | 1.Hubei Key Laboratory of Biologic Resources Protection and Utilization,Hubei Minzu University, Enshi 445000,China;2.College of Chemical and Environmental Engineering, Hubei Minzu University, Enshi 445000, China;3.Wulingshan Institute of Natural Medicine, Hubei University for Nationalities, Enshi 445000, China;4.Selenium Science and Industry Research Institute, Hubei Minzu University, Enshi 445000, China |
| DOI | DOI:10.19759/j.cnki.2164-4993.2025.06.014 |
| 年份 | 2025 |
| 刊期 | 6 |
| 页码 | 69-75,79 |
| 关键词 | Viburnum erosum Thunb.; Loganin; Ultrasonic-assisted extraction; Single-factor experiment; Response surface |
| 摘要 | [Objectives] This study was conducted to efficiently extract loganin from Viburnum erosum Thunb. [Methods] Ultrasound-assisted extraction coupled with response surface methodology was employed. The extraction was carried out with 60% methanol as the solvent and loganin extraction rate as the evaluation index. The effects of liquid-to-solid ratio, extraction temperature, and extraction time on loganin extraction rate were investigated through single-factor experiments. Subsequently, a Box-Behnken experimental design was implemented based on the single-factor results to optimize the extraction process. [Results] Under the conditions of liquid-to-solid ratio of 6 ml/g, extraction temperature of 39 ℃, and extraction time of 88 min, the loganin extraction rate was 4.199%, which agreed with the predicted value of 4.271%. The order of three factors affecting the extraction rate of loganin in V. erosum Thunb was liquid-to-solid ratio > extraction time > extraction temperature. The response surface methodology demonstrates high stability and feasibility for optimizing the extraction process, and the proposed protocol achieves efficient loganin extraction from V. erosum Thunb. [Conclusions] This study provides a novel approach for extracting iridoid glycosides from V. erosum Thunb. and expands the plant sources of loganin. |
