| 刊名 | Agricultural Biotechnology |
| 作者 | Dongli LIU1, Xuanqing KONG2, Xiaoming OU1,2, Zhenqing LIU1, WenjingYANG1, Jianhong LIU1* |
| 作者单位 | 1.College of Agriculture and Biotechnology, Hunan University of Humanities, Science and Technology, Loudi 417000, China; 2. National Engineering Research Center for Agrochemicals, Hunan Research Institute of Chemical Industry, Co., Ltd., Changsha 410014, China |
| DOI | DOI:10.19759/j.cnki.2164-4993.2025.06.010 |
| 年份 | 2025 |
| 刊期 | 6 |
| 页码 | 46-52 |
| 关键词 | Fluxapyroxad; Pyraclostrobin; Zebrafish; Joint toxicity; Mitochondrion; Oxidative stress |
| 摘要 | [Objectives] This study was conducted to investigate the joint toxicity of fungicides on aquatic ecosystems. [Methods] Using zebrafish as a model organism, an LC-MS/MS simultaneous detection method was established for fluxapyroxad and pyraclostrobin (with detection limits at ng/L level), and their acute toxicity, joint toxicity and toxic mechanisms were systematically evaluated. [Results] The toxicity of pyraclostrobin (96 h-LC₅₀ = 0.052 mg/L) to zebrafish was approximately 25.8 times higher than that of fluxapyroxad (96 h-LC₅₀ = 1.34 mg/L). Joint toxicity evaluation using the fixed-ratio ray design revealed that six of the seven mixture ratios exhibited additive effects (AI = 0.62-1.47), while the 8:1 ratio showed antagonism (AI = 2.14). The analysis of toxicity mechanisms indicated that both fungicides induced oxidative stress, lipid peroxidation, and cellular damage through inhibition of mitochondrial complex III and II, respectively, with pyraclostrobin inducing more pronounced hepatic MDA elevation (2.56-fold) and antioxidant enzyme inhibition. Ecological risk assessment demonstrated that fluxapyroxad posed moderate risk (RQ = 0.16-0.90), while pyraclostrobin posed moderate to high risk (RQ = 0.56-3.56), and crustaceans faced the highest risk. [Conclusions] This study elucidated the mechanism underlying toxicity differences due to distinct mitochondrial targets, providing a scientific basis for fungicide management. |
