| 刊名 | Agricultural Biotechnology |
| 作者 | Feng WEI1, Yan WANG1, Nana ZHAO2, Yu GUAN1, Weiqin MENG1, FENG LI1,Qingqing XU1,Changjiang WANG1* |
| 作者单位 | 1. Binzhou Institute of Animal Husbandry and Veterinary Science, Binzhou 256600, China; 2. Binzhou Center for Disease Control and Prevention, Binzhou 256600, China |
| DOI | DOI:10.19759/j.cnki.2164-4993.2025.02.008 |
| 年份 | 2025 |
| 刊期 | 2 |
| 页码 | 34-37,43 |
| 关键词 | Porcine circovirus type 2; Viral titer; IPMA; IFA |
| 摘要 | [Objectives] This study was conducted to find a convenient and reliable method for detecting the virus titer of porcine circovirus type 2 (PCV2). [Methods] The reaction conditions of the immunoperoxidase monolayer assay (IPMA) method for detecting the viral titer of PCV2 were optimized, and the results were compared with those obtained by indirect immunofluorescence assay (IFA). [Results] PCV2-infected cells exhibited brownish-red staining in either the nucleus or cytoplasm when detected by IPMA, and the detection results were largely consistent with IFA detection. [Conclusions] Both IPMA and IFA methods can be effectively used for determination of PCV2 viral titer, providing reliable support for assessing viral content during PCV2 vaccine development and validating virus inactivation efficacy. |
