| 刊名 | Agricultural Biotechnology |
| 作者 | Zhufang WU, Yong CHEN |
| 作者单位 | College of Agriculture and Biotechnology, Hunan University of Humanities, Science and Technology, Loudi 417000, China |
| DOI | DOI:10.19759/j.cnki.2164-4993.2025.02.002 |
| 年份 | 2025 |
| 刊期 | 2 |
| 页码 | 6-9,12 |
| 关键词 | Polygonatum sibiricum; Continuous cropping obstacle; Transcriptome; Differentially expressed gene; Functional analysis |
| 摘要 | [Objectives] This study was conducted to comprehensively understand the changes in gene expression of plants under environmental stress during different growth and development stages. [Methods] The effects of continuous cropping on the roots and leaves of Polygonatum sibiricum were investigated using transcriptome sequencing. Normally-grown first crop P. sibiricum was used as the control group, while continuous cropping plants served as the treatment group. Transcriptomic differences in roots and leaves under different conditions were compared. [Results] The leaf materials of first crop and continuous cropping P. sibiricum (CCLZ vs FCLZ) showed 21 916 differentially expressed genes (DEGs), while the root materials of first crop and continuous cropping P. sibiricum (CCRZ vs FCRZ) exhibited 12 726 DEGs (the lowest DEG count) (12 726). Among them, 1 896 DEGs were common. GO enrichment analysis revealed that DEGs were mainly enriched in metabolism, cell wall degradation, and pathogen defense. KEGG enrichment analysis indicated that DEGs in CCLZ vs FCLZ and CCRZ vs FCRZ primarily affected hormone signal transduction and pathogen interaction pathways. [Conclusions] This study preliminarily elucidates the regulatory mechanisms in the roots and leaves of continuous cropping P. sibiricum at the molecular level, providing reference for research on its adaptation to continuous cropping. |
