| 刊名 | Asian Agricultural Research |
| 作者 | Hongbing QI， Ziyang CHEN |
| 作者单位 | School of Life Science &Technology, Lingnan Normal University |
| DOI | 10.19601/j.cnki.issn1943-9903.2023.10.007 |
| 年份 | 2023 |
| 刊期 | 10 |
| 页码 | 23-27，36 |
| 关键词 | Aspergillus terreus, 6-Hydroxymellein synthase, Bioinformatics |
| 摘要 | [Objectives] To analyze the gene structure of the protein that predicts 6-hydroxymellein synthase of Aspergillus terreus and predict the characteristics and functions of the protein structure encoded by the gene. [Methods] Various information analysis tools in NCBI, CBS and ExPASy websites were adopted. [Results] The Q0D1P0 gene had a full length of 2 954 bp, with 952 amino acids in the coding area, and Q0D1P0 had the highest homology with the hypothetical protein ATETN484_0003008800. The molecular weight of Q0D1P0 protein was 105 040.56, the theoretical isoelectric point (pI) was 5.69 and the grand average of hydropathieity was -0.242. It was speculated that Q0D1P0 was an unstable and non-secretory hydrophilic protein located in cytoplasm without transmembrane domain or signal peptide. It could be predicted that the secondary structure of Q0D1P0 encoding protein consisted mainly of random coil, α-helix and a PKS-DH anhydrase domain. [Conclusions] The results will lay a theoretical foundation for cloning and expression of 6-hydroxymellein synthase and further understanding of its activity and function. |
