| 刊名 | Asian Agricultural Research |
| 作者 | Li LI， Shan JIANG， Tianmin CHE， Gang QIAO |
| 作者单位 | School of Life Sciences, Guizhou Normal University； School of International Education, Guizhou Normal University |
| DOI | 10.19601/j.cnki.issn1943-9903.2026.01.009 |
| 年份 | 2026 |
| 刊期 | 1 |
| 页码 | 51-57,64 |
| 关键词 | Physcomitrella patens, Lipoxygenase, Bioinformatics, Gene expression |
| 摘要 | [Objectives] To investigate the structure and function of the lipoxygenase (LOX) gene family in Physcomitrella patens. [Methods] This study employed bioinformatics methods to identify and predict LOX gene family members. Quantitative real-time PCR (qRT-PCR) was utilized to analyze the expression patterns of LOX genes at different stages of Botrytis cinerea infection. [Results] The P. patens LOX gene family comprises eight putative proteins, including two 12-LOX-type members and six 13-LOX-type members. Among the eight LOX proteins, PpLOX7 exhibited the lowest molecular weight and shortest amino acid sequence. PpLOX7 was identified as a basic protein with an isoelectric point (pI) of 8.54, while all other members were acidic. Subcellular localization analysis indicated that PpLOX7 was localized to the chloroplast, whereas the remaining members were distributed in the cytoplasm. Secondary structure prediction showed that all eight proteins were predominantly composed of random coils and α-helixes. Chromosomal mapping revealed that the LOX genes were distributed across 7 of the 27 chromosomes in P. patens, with PpLOX1 and PpLOX2 tandemly arranged on chromosome 15. The qRT-PCR analysis demonstrated distinct expression patterns among the eight PpLOX genes following B. cinerea infection. PpLOX1-3 and PpLOX7 were upregulated to varying degrees, suggesting their potential involvement in the early defense response of P. patens against B. cinerea. Notably, PpLOX2 exhibited highly significant differential expression, making it a key candidate for further investigation. [Conclusions] This study provides foundational insights into the functional roles of the LOX gene family in P. patens during biotic stress responses. |
