| 刊名 | Asian Agricultural Research |
| 作者 | Yunxia WANG， Xiaxia HOU， Xiaoli WU， Wang LIU |
| 作者单位 | Inner Mongolia Yili Industrial Group Co., Ltd.； Key Laboratory of Cattle and Sheep Milk and Meat Products Risk Control and Key Technology, State Administration for Market Regulation |
| DOI | 10.19601/j.cnki.issn1943-9903.2026.01.008 |
| 年份 | 2026 |
| 刊期 | 1 |
| 页码 | 46-50,60 |
| 关键词 | Staphylococcus aureus, Chip digital PCR (cdPCR), Sensitivity |
| 摘要 | [Objectives] This study aimed to evaluate the detection sensitivity of Staphylococcus aureus in dairy products utilizing the chip digital PCR (cdPCR) technique. [Methods] Specific primers and probes were designed and synthesized based on the conserved sequence of the heat-resistant nuclease gene nuc of S. aureus. cdPCR was employed to detect S. aureus, and the sensitivity of this technique was systematically assessed in samples exhibiting low levels of contamination. [Results] cdPCR demonstrated precise quantification when the initial concentration of the sample enrichment solution was equal to or greater than 50 CFU/mL. The detection dynamic range extended across at least five orders of magnitude, with a minimum DNA detection limit of 0.230 4 pg/μL. In artificially contaminated cheese samples, the method’s lower limit of quantification for detecting S. aureus was 8×102 CFU/g. Regression analysis demonstrated that the gene copy number concentration measured by cdPCR exhibited a strong linear correlation with bacterial contamination concentration across a broad range. [Conclusions] The cdPCR method developed in this study demonstrates high sensitivity and robust quantitative capabilities, offering a reliable technical approach for the precise detection of low-level S. aureus contamination in dairy products. |
