| 刊名 | Asian Agricultural Research |
| 作者 | Litao LI， Wenshi ZHAO， Haijiao XU， Changxin XU， Rui JIAO， Liming HE |
| 作者单位 | Changli Institute of Pomology, Hebei Academy of Agriculture and Forestry Sciences |
| DOI | 10.19601/j.cnki.issn1943-9903.2025.12.011 |
| 年份 | 2025 |
| 刊期 | 12 |
| 页码 | 53-55 |
| 关键词 | Pyemotes zhonghuajia, Entomopathogenic fungi, Vector behavior, Biological control, Dynamic changes |
| 摘要 | [Objectives] This study aimed to quantitatively investigate the dynamic patterns of spore powder of Metarhizium anisopliae and Beauveria bassiana carried on the body surface of Pyemotes zhonghuajia, providing a theoretical basis for optimizing synergistic biological control strategies utilizing natural enemies and pathogens. [Methods] Under laboratory conditions, the spore load on mites after crawling on a spore-powder-coated surface for different durations (0-4 h), and the spore shedding after crawling on a clean surface for different durations (0-4 h) post-loading were measured. [Results] The spore load of mites for both fungi increased with crawling time, but exhibited distinct kinetic patterns. The load of M. anisopliae spores peaked at 2 h (6.86×104 spores/mite) and then decreased significantly; whereas the load of B. bassiana spores continued to increase to a maximum (12.83×104 spores/mite) within 4 h, with the rate of increase slowing significantly after 2 h. During the shedding phase, the number of both types of spores decreased with crawling time. After 4 h, the residual amount of B. bassiana spores (3.18×104 spores/mite) was significantly higher than that of M. anisopliae (2.51×104 spores/mite). [Conclusions] The process of P. zhonghuajia carrying entomopathogenic fungal spores exhibits significant temporal dynamics and species specificity. The findings identify key time points for spore loading and shedding, providing crucial parameters for determining the optimal pre-release "loading" duration and assessing the mites’ sustained dispersal capacity in the field, which holds significant importance for advancing the application of synergistic biological control technology. |
