| 刊名 | Asian Agricultural Research |
| 作者 | Fangfang DA， Yufeng CHEN， Ziwan YUAN， Ying LIU， Yaoting MENG， Kequn HE， Yanmin XIE |
| 作者单位 | College of Ethnic Medicine，Guizhou Minzu University； College of Pharmacy |
| DOI | 10.19601/j.cnki.issn1943-9903.2025.10.006 |
| 年份 | 2025 |
| 刊期 | 10 |
| 页码 | 28-31，44 |
| 关键词 | Tetracera asiatica,High-performance liquid chromatography (HPLC),Components,Quantitative determination |
| 摘要 | [Objectives]To establish an HPLC method for the quantitative determination of multiple phenolic acid components in Tetracera asiatica medicinal material,providing a basis for establishing its quality standards.[Methods]An Inertsil ODS-C18 column (250 mm×4.6 mm,5 μm) was used.The mobile phase consisted of acetonitrile-0.2% phosphoric acid solution (10：90).The flow rate was 1.0 mL/min.The detection wavelength was 274 nm.The column temperature was 25 ℃.The injection volume was 10 μL.The content of three components,gallic acid,protocatechuic acid,and protocatechualdehyde,was determined in 13 batches of T. asiatica.[Results]Gallic acid showed good linearity within the range of 0.020-6.400 μg/mL,protocatechuic acid within 0.201-6.432 μg/mL,and protocatechualdehyde within 0.202-6.464 μg/mL (r>0.999 0).The average recovery rates ranged from 98.61% to 101.17%,with RSDs between 1.21% and 2.69%.[Conclusions]The quantitative determination method established in this study is simple and feasible,and can provide a basis for the quality evaluation of T. asiatica. |
